Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3974198..3974892 Replicon chromosome
Accession NZ_CP023364
Organism Escherichia coli strain 144

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag CNQ53_RS20995 Protein ID WP_001263491.1
Coordinates 3974198..3974596 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag CNQ53_RS21000 Protein ID WP_000554755.1
Coordinates 3974599..3974892 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ53_RS20965 3969367..3970611 - 1245 WP_000189541.1 esterase FrsA -
- 3970027..3970107 - 81 NuclAT_11 - -
- 3970027..3970107 - 81 NuclAT_11 - -
- 3970027..3970107 - 81 NuclAT_11 - -
- 3970027..3970107 - 81 NuclAT_11 - -
CNQ53_RS20970 3970703..3971161 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
CNQ53_RS20975 3971422..3972879 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
CNQ53_RS20980 3972936..3973288 - 353 Protein_3671 peptide chain release factor H -
CNQ53_RS20985 3973284..3973490 - 207 Protein_3672 RtcB family protein -
CNQ53_RS20990 3973736..3974188 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
CNQ53_RS20995 3974198..3974596 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CNQ53_RS21000 3974599..3974892 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CNQ53_RS21005 3974944..3975999 - 1056 WP_001550655.1 DNA polymerase IV -
CNQ53_RS21010 3976070..3976855 - 786 WP_019842510.1 putative lateral flagellar export/assembly protein LafU -
CNQ53_RS21015 3976827..3978539 + 1713 Protein_3678 flagellar biosynthesis protein FlhA -
CNQ53_RS21020 3978644..3978922 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CNQ53_RS21025 3978915..3979271 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3964134..3974892 10758


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T80945 WP_001263491.1 NZ_CP023364:c3974596-3974198 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T80945 NZ_CP023364:c3974596-3974198 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT80945 WP_000554755.1 NZ_CP023364:c3974892-3974599 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT80945 NZ_CP023364:c3974892-3974599 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References