Detailed information of TA system TA080920

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 39474..39738
• Replicon: plasmid p85
• Accession: NZ_CP023362
• Organism: Escherichia coli strain 1943

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: CNQ54_RS25525
• Protein ID: WP_001387489.1
• Coordinates: 39586..39738 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	39474..39534 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ54_RS25500	34546..35754	-	1209	WP_011264049.1	IncI1-type conjugal transfer protein TrbA	-
-	35934..35991	-	58	NuclAT_1	-	-
-	35934..35991	-	58	NuclAT_1	-	-
-	35934..35991	-	58	NuclAT_1	-	-
-	35934..35991	-	58	NuclAT_1	-	-
CNQ54_RS25505	36061..36810	-	750	Protein_44	protein finQ	-
CNQ54_RS25510	37039..37356	+	318	WP_000118520.1	quaternary ammonium compound efflux SMR transporter SugE	-
CNQ54_RS25515	37353..37886	-	534	WP_001221666.1	lipocalin family protein	-
CNQ54_RS25520	37980..39125	-	1146	WP_000976514.1	class C beta-lactamase CMY-2	-
-	39474..39534	-	61	NuclAT_0	-	Antitoxin
-	39474..39534	-	61	NuclAT_0	-	Antitoxin
-	39474..39534	-	61	NuclAT_0	-	Antitoxin
-	39474..39534	-	61	NuclAT_0	-	Antitoxin
CNQ54_RS25525	39586..39738	+	153	WP_001387489.1	Hok/Gef family protein	Toxin
CNQ54_RS25530	39810..40061	-	252	WP_001291964.1	hypothetical protein	-
CNQ54_RS25535	40361..40657	+	297	WP_011264046.1	hypothetical protein	-
CNQ54_RS26295	40722..40898	-	177	WP_001054897.1	hypothetical protein	-
CNQ54_RS25540	41290..41499	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
CNQ54_RS25545	41571..42233	-	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
CNQ54_RS25550	42304..44472	-	2169	WP_023518847.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-2	-	1..85570	85570

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T80920 WP_001387489.1 NZ_CP023362:39586-39738 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T80920 NZ_CP023362:39586-39738 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT80920 NZ_CP023362:c39534-39474 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin