Detailed information of TA system TA080916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4626240..4626842
• Replicon: chromosome
• Accession: NZ_CP023359
• Organism: Escherichia coli strain 1943

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CNQ54_RS23635
• Protein ID: WP_000897305.1
• Coordinates: 4626531..4626842 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CNQ54_RS23630
• Protein ID: WP_000356397.1
• Coordinates: 4626240..4626530 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ54_RS23600	4622166..4623068	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CNQ54_RS23605	4623065..4623700	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CNQ54_RS23610	4623697..4624626	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CNQ54_RS23615	4624956..4625198	-	243	Protein_4331	hypothetical protein	-
CNQ54_RS23620	4625417..4625635	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CNQ54_RS23630	4626240..4626530	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CNQ54_RS23635	4626531..4626842	-	312	WP_000897305.1	hypothetical protein	Toxin
CNQ54_RS23640	4627071..4627979	+	909	WP_001162704.1	alpha/beta hydrolase	-
CNQ54_RS23645	4628043..4628984	-	942	WP_157733343.1	fatty acid biosynthesis protein FabY	-
CNQ54_RS23650	4629029..4629466	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CNQ54_RS23655	4629463..4630335	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CNQ54_RS23660	4630329..4630928	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T80916 WP_000897305.1 NZ_CP023359:c4626842-4626531 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T80916 NZ_CP023359:c4626842-4626531 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT80916 WP_000356397.1 NZ_CP023359:c4626530-4626240 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT80916 NZ_CP023359:c4626530-4626240 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7