Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3801460..3802001 Replicon chromosome
Accession NZ_CP023359
Organism Escherichia coli strain 1943

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag CNQ54_RS19600 Protein ID WP_000615976.1
Coordinates 3801723..3802001 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CNQ54_RS19595 Protein ID WP_000729704.1
Coordinates 3801460..3801720 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ54_RS19570 3796507..3797646 + 1140 WP_053289132.1 flagellar type III secretion system protein FlhB -
CNQ54_RS19575 3797630..3799712 + 2083 Protein_3604 flagellar biosynthesis protein FlhA -
CNQ54_RS19580 3799817..3800095 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CNQ54_RS19585 3800088..3800444 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
CNQ54_RS19590 3800501..3801274 - 774 WP_000543895.1 C40 family peptidase -
CNQ54_RS19595 3801460..3801720 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CNQ54_RS19600 3801723..3802001 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CNQ54_RS19605 3802157..3802897 + 741 WP_001225679.1 murein L,D-transpeptidase -
CNQ54_RS19610 3802868..3803635 - 768 WP_000333380.1 class II glutamine amidotransferase -
CNQ54_RS19615 3803841..3804419 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3799817..3812787 12970


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T80914 WP_000615976.1 NZ_CP023359:3801723-3802001 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T80914 NZ_CP023359:3801723-3802001 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT80914 WP_000729704.1 NZ_CP023359:3801460-3801720 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT80914 NZ_CP023359:3801460-3801720 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References