Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1813937..1814439 Replicon chromosome
Accession NZ_CP023359
Organism Escherichia coli strain 1943

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CNQ54_RS09145 Protein ID WP_000767829.1
Coordinates 1814185..1814439 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CNQ54_RS09140 Protein ID WP_001259255.1
Coordinates 1813937..1814188 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ54_RS09110 1809115..1810182 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CNQ54_RS09115 1810182..1811252 - 1071 WP_096035014.1 histidinol-phosphate transaminase -
CNQ54_RS09120 1811249..1812553 - 1305 WP_032283328.1 histidinol dehydrogenase -
CNQ54_RS09125 1812559..1813458 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CNQ54_RS09135 1813604..1813654 - 51 WP_001364200.1 his operon leader peptide -
CNQ54_RS09140 1813937..1814188 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CNQ54_RS09145 1814185..1814439 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CNQ54_RS09150 1814522..1815346 + 825 WP_000754737.1 SDR family oxidoreductase -
CNQ54_RS09155 1815392..1816321 + 930 WP_032283327.1 LysR family transcriptional regulator -
CNQ54_RS25925 1816536..1816598 + 63 WP_010723108.1 membrane protein YoeI -
CNQ54_RS09160 1816588..1817946 + 1359 WP_024198437.1 putrescine/proton symporter PlaP -
CNQ54_RS09165 1818125..1819183 + 1059 WP_096035015.1 transport protein YeeE -
CNQ54_RS09170 1819197..1819424 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T80902 WP_000767829.1 NZ_CP023359:1814185-1814439 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T80902 NZ_CP023359:1814185-1814439 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT80902 WP_001259255.1 NZ_CP023359:1813937-1814188 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT80902 NZ_CP023359:1813937-1814188 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References