Detailed information of TA system TA080859

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 71388..71641
• Replicon: plasmid p72
• Accession: NZ_CP023355
• Organism: Escherichia coli strain 746

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: CNQ56_RS26885
• Protein ID: WP_001312851.1
• Coordinates: 71492..71641 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	71388..71444 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ56_RS26845	67883..68638	+	756	Protein_85	conjugal transfer pilus acetylase TraX	-
CNQ56_RS26850	68695..69250	+	556	Protein_86	fertility inhibition protein FinO	-
CNQ56_RS26855	69393..69612	+	220	Protein_87	hypothetical protein	-
CNQ56_RS26860	69859..70321	+	463	Protein_88	thermonuclease family protein	-
CNQ56_RS26865	70396..70583	+	188	Protein_89	hemolysin expression modulator Hha	-
CNQ56_RS26870	70623..71213	+	591	WP_096035224.1	DUF2726 domain-containing protein	-
-	71388..71444	-	57	NuclAT_1	-	Antitoxin
-	71388..71444	-	57	NuclAT_1	-	Antitoxin
-	71388..71444	-	57	NuclAT_1	-	Antitoxin
-	71388..71444	-	57	NuclAT_1	-	Antitoxin
CNQ56_RS26885	71492..71641	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
CNQ56_RS26890	71925..72182	+	258	WP_000083826.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..72470	72470

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T80859 WP_001312851.1 NZ_CP023355:71492-71641 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T80859 NZ_CP023355:71492-71641 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT80859 NZ_CP023355:c71444-71388 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin