Detailed information of TA system TA080856

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27608..27877
• Replicon: plasmid p72
• Accession: NZ_CP023355
• Organism: Escherichia coli strain 746

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CNQ56_RS26565
• Protein ID: WP_001312861.1
• Coordinates: 27719..27877 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	27608..27673 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ56_RS28120	23335..23453	+	119	Protein_32	single-stranded DNA-binding protein	-
CNQ56_RS26525	23483..23842	+	360	Protein_33	single-stranded DNA-binding protein	-
CNQ56_RS26530	23949..24188	+	240	WP_096035201.1	DUF905 family protein	-
CNQ56_RS26545	24255..26210	+	1956	Protein_35	ParB/RepB/Spo0J family partition protein	-
CNQ56_RS26550	26284..26723	+	440	Protein_36	conjugation system SOS inhibitor PsiB	-
CNQ56_RS26555	26720..27439	+	720	WP_096035202.1	plasmid SOS inhibition protein A	-
CNQ56_RS26560	27451..27639	-	189	WP_001299721.1	hypothetical protein	-
-	27451..27675	+	225	NuclAT_0	-	-
-	27451..27675	+	225	NuclAT_0	-	-
-	27451..27675	+	225	NuclAT_0	-	-
-	27451..27675	+	225	NuclAT_0	-	-
-	27608..27673	+	66	-	-	Antitoxin
CNQ56_RS26565	27719..27877	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CNQ56_RS28125	28568..28774	+	207	WP_000275856.1	hypothetical protein	-
CNQ56_RS26580	28798..29100	+	303	WP_001272235.1	hypothetical protein	-
CNQ56_RS26590	29256..29543	+	288	WP_000107542.1	hypothetical protein	-
CNQ56_RS26595	29662..30483	+	822	WP_157739658.1	DUF945 domain-containing protein	-
CNQ56_RS26600	30780..31382	-	603	WP_096035204.1	transglycosylase SLT domain-containing protein	-
CNQ56_RS26610	31705..32088	+	384	WP_096035227.1	relaxosome protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..72470	72470

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T80856 WP_001312861.1 NZ_CP023355:27719-27877 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T80856 NZ_CP023355:27719-27877 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT80856 NZ_CP023355:27608-27673 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin