Detailed information of TA system TA080852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4925758..4926360
• Replicon: chromosome
• Accession: NZ_CP023353
• Organism: Escherichia coli strain 746

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CNQ56_RS24995
• Protein ID: WP_000897305.1
• Coordinates: 4926049..4926360 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CNQ56_RS24990
• Protein ID: WP_000356395.1
• Coordinates: 4925758..4926048 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ56_RS24955	4921382..4922284	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CNQ56_RS24960	4922281..4922916	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CNQ56_RS24965	4922913..4923842	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
CNQ56_RS24970	4924024..4924266	-	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
CNQ56_RS24975	4924485..4924703	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CNQ56_RS24980	4925122..4925400	-	279	WP_001315112.1	hypothetical protein	-
CNQ56_RS24985	4925452..4925673	-	222	WP_001550354.1	hypothetical protein	-
CNQ56_RS24990	4925758..4926048	-	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
CNQ56_RS24995	4926049..4926360	-	312	WP_000897305.1	hypothetical protein	Toxin
CNQ56_RS25000	4926589..4927497	+	909	WP_001550353.1	alpha/beta hydrolase	-
CNQ56_RS25010	4927665..4929479	-	1815	WP_001550352.1	hypothetical protein	-
CNQ56_RS25015	4929895..4930836	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CNQ56_RS25020	4930881..4931318	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T80852 WP_000897305.1 NZ_CP023353:c4926360-4926049 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T80852 NZ_CP023353:c4926360-4926049 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT80852 WP_000356395.1 NZ_CP023353:c4926048-4925758 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT80852 NZ_CP023353:c4926048-4925758 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7