Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3970428..3971122 Replicon chromosome
Accession NZ_CP023353
Organism Escherichia coli strain 746

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag CNQ56_RS20155 Protein ID WP_001263491.1
Coordinates 3970428..3970826 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag CNQ56_RS20160 Protein ID WP_000554755.1
Coordinates 3970829..3971122 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CNQ56_RS20125 3965597..3966841 - 1245 WP_000189541.1 esterase FrsA -
- 3966257..3966337 - 81 NuclAT_10 - -
- 3966257..3966337 - 81 NuclAT_10 - -
- 3966257..3966337 - 81 NuclAT_10 - -
- 3966257..3966337 - 81 NuclAT_10 - -
CNQ56_RS20130 3966933..3967391 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
CNQ56_RS20135 3967652..3969109 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
CNQ56_RS20140 3969166..3969518 - 353 Protein_3678 peptide chain release factor H -
CNQ56_RS20145 3969514..3969720 - 207 Protein_3679 RtcB family protein -
CNQ56_RS20150 3969966..3970418 - 453 WP_019842500.1 GNAT family N-acetyltransferase -
CNQ56_RS20155 3970428..3970826 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CNQ56_RS20160 3970829..3971122 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CNQ56_RS20165 3971174..3972229 - 1056 WP_001550655.1 DNA polymerase IV -
CNQ56_RS20170 3972300..3973085 - 786 WP_074157994.1 putative lateral flagellar export/assembly protein LafU -
CNQ56_RS20175 3973057..3974769 + 1713 Protein_3685 flagellar biosynthesis protein FlhA -
CNQ56_RS20180 3974874..3975152 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CNQ56_RS20185 3975145..3975501 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE 3919542..3971122 51580


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T80845 WP_001263491.1 NZ_CP023353:c3970826-3970428 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T80845 NZ_CP023353:c3970826-3970428 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT80845 WP_000554755.1 NZ_CP023353:c3971122-3970829 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT80845 NZ_CP023353:c3971122-3970829 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References