Detailed information of TA system TA080829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1265121..1265848
• Replicon: chromosome
• Accession: NZ_CP023353
• Organism: Escherichia coli strain 746

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0YLE2
• Locus tag: CNQ56_RS06480
• Protein ID: WP_000547555.1
• Coordinates: 1265121..1265432 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CNQ56_RS06485
• Protein ID: WP_000126294.1
• Coordinates: 1265429..1265848 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CNQ56_RS06455	1261056..1262765	+	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-
CNQ56_RS06460	1262775..1263317	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
CNQ56_RS06465	1263317..1264084	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
CNQ56_RS06470	1264081..1264491	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
CNQ56_RS06475	1264484..1264954	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
CNQ56_RS06480	1265121..1265432	+	312	WP_000547555.1	type II toxin-antitoxin system HigB family toxin	Toxin
CNQ56_RS06485	1265429..1265848	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
CNQ56_RS06490	1265927..1267351	-	1425	WP_000110355.1	6-phospho-beta-glucosidase AscB	-
CNQ56_RS06495	1267360..1268817	-	1458	WP_001107889.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
CNQ56_RS06500	1269077..1270087	+	1011	WP_029130985.1	DNA-binding transcriptional regulator AscG	-
CNQ56_RS06505	1270236..1270763	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12439.18 Da
• Isoelectric Point: 9.5146

>T80829 WP_000547555.1 NZ_CP023353:1265121-1265432 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T80829 NZ_CP023353:1265121-1265432 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT80829 WP_000126294.1 NZ_CP023353:1265429-1265848 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT80829 NZ_CP023353:1265429-1265848 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V0YLE2