Detailed information of TA system TA080819

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 95790..96054
• Replicon: plasmid unnamed1
• Accession: NZ_CP023350
• Organism: Escherichia coli strain ETEC-2264

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: -
• Locus tag: CJU63_RS26425
• Protein ID: WP_001704300.1
• Coordinates: 95790..95942 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	95992..96054 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJU63_RS26400	91272..93440	+	2169	WP_001388820.1	IncI1-type conjugal transfer membrane protein TraY	-
CJU63_RS26405	93516..94114	+	599	Protein_103	plasmid IncI1-type surface exclusion protein ExcA	-
CJU63_RS26410	94212..94421	+	210	WP_000416064.1	hemolysin expression modulator Hha	-
CJU63_RS27990	94630..94806	+	177	WP_001054897.1	hypothetical protein	-
CJU63_RS26415	94871..95167	-	297	WP_001275298.1	hypothetical protein	-
CJU63_RS26420	95467..95718	+	252	WP_001291963.1	hypothetical protein	-
CJU63_RS26425	95790..95942	-	153	WP_001704300.1	Hok/Gef family protein	Toxin
-	95992..96054	+	63	NuclAT_0	-	Antitoxin
-	95992..96054	+	63	NuclAT_0	-	Antitoxin
-	95992..96054	+	63	NuclAT_0	-	Antitoxin
-	95992..96054	+	63	NuclAT_0	-	Antitoxin
-	96295..96350	+	56	NuclAT_1	-	-
-	96295..96350	+	56	NuclAT_1	-	-
-	96295..96350	+	56	NuclAT_1	-	-
-	96295..96350	+	56	NuclAT_1	-	-
CJU63_RS26430	96511..97497	+	987	WP_000987364.1	hypothetical protein	-
CJU63_RS26435	97714..98922	+	1209	WP_001388819.1	IncI1-type conjugal transfer protein TrbA	-
CJU63_RS26440	98941..100011	+	1071	WP_000151586.1	IncI1-type conjugal transfer protein TrbB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	eltA / eltB / csvA	1..112045	112045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5809.14 Da
• Isoelectric Point: 8.7948

>T80819 WP_001704300.1 NZ_CP023350:c95942-95790 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVFVATLAYEVKR

Nucleotide

• Length: 153 bp

>T80819 NZ_CP023350:c95942-95790 [Escherichia coli]
ATGCCACAGCGAACATTTTTAATGATGTTGATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGTTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT80819 NZ_CP023350:95992-96054 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Antitoxin