Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2243858..2244360 Replicon chromosome
Accession NZ_CP023349
Organism Escherichia coli strain ETEC-2264

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CJU63_RS11745 Protein ID WP_000767829.1
Coordinates 2243858..2244112 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CJU63_RS11750 Protein ID WP_001259255.1
Coordinates 2244109..2244360 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJU63_RS11720 2238873..2239100 - 228 WP_000985280.1 sulfurtransferase TusA family protein -
CJU63_RS11725 2239114..2240172 - 1059 WP_000492318.1 transport protein YeeE -
CJU63_RS11730 2240351..2241709 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CJU63_RS27545 2241699..2241761 - 63 WP_010723108.1 membrane protein YoeI -
CJU63_RS11735 2241976..2242905 - 930 WP_000803351.1 LysR family transcriptional regulator -
CJU63_RS11740 2242951..2243775 - 825 WP_000754758.1 SDR family oxidoreductase -
CJU63_RS11745 2243858..2244112 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CJU63_RS11750 2244109..2244360 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CJU63_RS11755 2244643..2244693 + 51 WP_001364200.1 his operon leader peptide -
CJU63_RS11765 2244839..2245738 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CJU63_RS11770 2245744..2247048 + 1305 WP_001356062.1 histidinol dehydrogenase -
CJU63_RS11775 2247045..2248115 + 1071 WP_000108967.1 histidinol-phosphate transaminase -
CJU63_RS11780 2248115..2249182 + 1068 WP_000080130.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T80808 WP_000767829.1 NZ_CP023349:c2244112-2243858 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T80808 NZ_CP023349:c2244112-2243858 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT80808 WP_001259255.1 NZ_CP023349:c2244360-2244109 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT80808 NZ_CP023349:c2244360-2244109 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References