Detailed information of TA system TA080787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4441654..4442256
• Replicon: chromosome
• Accession: NZ_CP023346
• Organism: Escherichia coli strain ETEC-2265

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CJU64_RS23110
• Protein ID: WP_000897305.1
• Coordinates: 4441654..4441965 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CJU64_RS23115
• Protein ID: WP_000356397.1
• Coordinates: 4441966..4442256 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJU64_RS23080	4436684..4437469	+	786	WP_001662158.1	DeoR/GlpR family DNA-binding transcription regulator	-
CJU64_RS23085	4437568..4438167	+	600	WP_001295269.1	glucose-1-phosphatase	-
CJU64_RS23090	4438161..4439033	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CJU64_RS23095	4439030..4439467	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CJU64_RS23100	4439512..4440453	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CJU64_RS23105	4440517..4441425	-	909	WP_001162704.1	alpha/beta hydrolase	-
CJU64_RS23110	4441654..4441965	+	312	WP_000897305.1	hypothetical protein	Toxin
CJU64_RS23115	4441966..4442256	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CJU64_RS23125	4442861..4443079	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CJU64_RS23130	4443298..4443540	+	243	WP_001086388.1	hypothetical protein	-
CJU64_RS23135	4443870..4444799	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CJU64_RS23140	4444796..4445431	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CJU64_RS23145	4445428..4446330	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T80787 WP_000897305.1 NZ_CP023346:4441654-4441965 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T80787 NZ_CP023346:4441654-4441965 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT80787 WP_000356397.1 NZ_CP023346:4441966-4442256 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT80787 NZ_CP023346:4441966-4442256 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7