Detailed information of TA system TA080785

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3595176..3595975
• Replicon: chromosome
• Accession: NZ_CP023346
• Organism: Escherichia coli strain ETEC-2265

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: U9XVR9
• Locus tag: CJU64_RS18690
• Protein ID: WP_000347267.1
• Coordinates: 3595511..3595975 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: CJU64_RS18685
• Protein ID: WP_001307405.1
• Coordinates: 3595176..3595511 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJU64_RS18670	3590961..3591731	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
CJU64_RS18675	3591747..3593081	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
CJU64_RS18680	3593456..3595027	+	1572	WP_001273741.1	galactarate dehydratase	-
CJU64_RS18685	3595176..3595511	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CJU64_RS18690	3595511..3595975	+	465	WP_000347267.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CJU64_RS18695	3596030..3596839	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CJU64_RS18705	3597088..3598368	+	1281	WP_000681921.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CJU64_RS18710	3598391..3598864	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CJU64_RS18715	3598875..3599654	+	780	WP_000406212.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CJU64_RS18720	3599644..3600522	+	879	WP_001355782.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CJU64_RS18725	3600540..3600974	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3584301..3595975	11674

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17807.17 Da
• Isoelectric Point: 9.4947

>T80785 WP_000347267.1 NZ_CP023346:3595511-3595975 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T80785 NZ_CP023346:3595511-3595975 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT80785 WP_001307405.1 NZ_CP023346:3595176-3595511 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT80785 NZ_CP023346:3595176-3595511 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0E0XTR4

Antitoxin

• AlphaFold DB: A0A0E0XVC7