Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2294908..2295410 Replicon chromosome
Accession NZ_CP023346
Organism Escherichia coli strain ETEC-2265

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CJU64_RS12145 Protein ID WP_000767829.1
Coordinates 2294908..2295162 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CJU64_RS12150 Protein ID WP_001259255.1
Coordinates 2295159..2295410 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJU64_RS12120 2289923..2290150 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CJU64_RS12125 2290164..2291222 - 1059 WP_000492305.1 transport protein YeeE -
CJU64_RS12130 2291401..2292759 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CJU64_RS28405 2292749..2292811 - 63 WP_010723108.1 membrane protein YoeI -
CJU64_RS12135 2293026..2293955 - 930 WP_000803360.1 LysR family transcriptional regulator -
CJU64_RS12140 2294001..2294825 - 825 WP_000754737.1 SDR family oxidoreductase -
CJU64_RS12145 2294908..2295162 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CJU64_RS12150 2295159..2295410 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CJU64_RS12155 2295693..2295743 + 51 WP_001364200.1 his operon leader peptide -
CJU64_RS12165 2295889..2296788 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CJU64_RS12170 2296794..2298098 + 1305 WP_001355813.1 histidinol dehydrogenase -
CJU64_RS12175 2298095..2299165 + 1071 WP_001099220.1 histidinol-phosphate transaminase -
CJU64_RS12180 2299165..2300232 + 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T80780 WP_000767829.1 NZ_CP023346:c2295162-2294908 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T80780 NZ_CP023346:c2295162-2294908 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT80780 WP_001259255.1 NZ_CP023346:c2295410-2295159 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT80780 NZ_CP023346:c2295410-2295159 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References