Detailed information of TA system TA080758

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1261043..1261268
• Replicon: chromosome
• Accession: NZ_CP023346
• Organism: Escherichia coli strain ETEC-2265

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: CJU64_RS06440
• Protein ID: WP_000813254.1
• Coordinates: 1261113..1261268 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1261043..1261101 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJU64_RS06400	1256353..1256580	+	228	WP_000476985.1	transcriptional regulator	-
CJU64_RS06405	1256564..1257085	+	522	WP_000705382.1	hypothetical protein	-
CJU64_RS06410	1257066..1258031	+	966	WP_000054517.1	hypothetical protein	-
CJU64_RS06415	1258037..1258783	+	747	WP_000788772.1	ATP-binding protein	-
CJU64_RS06420	1258805..1259566	+	762	WP_000450703.1	DUF1627 domain-containing protein	-
CJU64_RS06425	1259599..1259880	+	282	WP_001509842.1	hypothetical protein	-
CJU64_RS06430	1259883..1260295	+	413	Protein_1166	DUF4406 domain-containing protein	-
CJU64_RS06435	1260399..1260821	+	423	WP_000160146.1	hypothetical protein	-
-	1261043..1261101	-	59	-	-	Antitoxin
CJU64_RS06440	1261113..1261268	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
CJU64_RS06445	1261484..1261663	+	180	WP_001277775.1	hypothetical protein	-
CJU64_RS06450	1261682..1262167	+	486	WP_000818165.1	type II toxin-antitoxin system death-on-curing family toxin	-
CJU64_RS06455	1262368..1262628	+	261	WP_000701918.1	hypothetical protein	-
CJU64_RS06460	1262695..1262973	+	279	WP_023607105.1	hypothetical protein	-
CJU64_RS06465	1262975..1264030	+	1056	WP_001502278.1	DUF968 domain-containing protein	-
CJU64_RS06470	1264031..1264396	+	366	WP_001297842.1	RusA family crossover junction endodeoxyribonuclease	-
CJU64_RS06475	1264405..1264947	+	543	WP_001398904.1	DUF1133 family protein	-
CJU64_RS06480	1265260..1265457	+	198	WP_000917767.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1242411..1298913	56502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T80758 WP_000813254.1 NZ_CP023346:1261113-1261268 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T80758 NZ_CP023346:1261113-1261268 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTTATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT80758 NZ_CP023346:c1261101-1261043 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin