Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5841258..5841853 Replicon chromosome
Accession NZ_CP023316
Organism Pseudomonas aeruginosa strain PPF-1

Toxin (Protein)


Gene name higB Uniprot ID V6ALY3
Locus tag B8B76_RS27890 Protein ID WP_003113526.1
Coordinates 5841575..5841853 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0S3KUN4
Locus tag B8B76_RS27885 Protein ID WP_003111575.1
Coordinates 5841258..5841563 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B8B76_RS27845 5836326..5836802 - 477 WP_003125367.1 putative molybdenum carrier protein -
B8B76_RS27850 5836805..5837215 - 411 WP_003125368.1 hypothetical protein -
B8B76_RS27855 5837212..5837793 - 582 WP_202803835.1 hypothetical protein -
B8B76_RS27860 5837874..5838413 - 540 WP_202803836.1 hypothetical protein -
B8B76_RS27865 5838683..5838907 + 225 WP_042956132.1 helix-turn-helix domain-containing protein -
B8B76_RS27870 5839332..5840354 - 1023 WP_003125371.1 hypothetical protein -
B8B76_RS27875 5840393..5840605 - 213 WP_003125372.1 AlpA family phage regulatory protein -
B8B76_RS27885 5841258..5841563 - 306 WP_003111575.1 HigA family addiction module antidote protein Antitoxin
B8B76_RS27890 5841575..5841853 - 279 WP_003113526.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B8B76_RS27900 5842182..5844410 + 2229 WP_021264080.1 TonB-dependent receptor -
B8B76_RS27905 5844480..5845127 - 648 WP_003095021.1 carbonate dehydratase -
B8B76_RS27910 5845189..5846427 - 1239 WP_003111578.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(6-82)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10646.19 Da        Isoelectric Point: 7.8937

>T80749 WP_003113526.1 NZ_CP023316:c5841853-5841575 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T80749 NZ_CP023316:c5841853-5841575 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACTTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11205.72 Da        Isoelectric Point: 4.4475

>AT80749 WP_003111575.1 NZ_CP023316:c5841563-5841258 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGEQIEHEIEPLLAQG

Download         Length: 306 bp

>AT80749 NZ_CP023316:c5841563-5841258 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCGAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCAGGGATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V6ALY3

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3KUN4

References