Detailed information of TA system TA080738

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3053986..3054620
• Replicon: chromosome
• Accession: NZ_CP023315
• Organism: Caulobacter vibrioides strain CB13b1a

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: CA606_RS14425
• Protein ID: WP_096050496.1
• Coordinates: 3053986..3054273 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CA606_RS14430
• Protein ID: WP_096050495.1
• Coordinates: 3054306..3054620 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA606_RS14390	3049079..3049204	+	126	WP_096050503.1	hypothetical protein	-
CA606_RS14395	3049201..3050169	+	969	WP_096050502.1	glycosyl transferase	-
CA606_RS14400	3050482..3051333	+	852	WP_096050501.1	SDR family NAD(P)-dependent oxidoreductase	-
CA606_RS14405	3051335..3052108	-	774	WP_096050500.1	sulfite exporter TauE/SafE family protein	-
CA606_RS14410	3052113..3052649	-	537	WP_096050499.1	rhodanese family protein	-
CA606_RS14415	3052615..3052977	-	363	WP_181242904.1	metalloregulator ArsR/SmtB family transcription factor	-
CA606_RS14420	3053051..3053911	+	861	WP_096050497.1	MBL fold metallo-hydrolase	-
CA606_RS14425	3053986..3054273	+	288	WP_096050496.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CA606_RS14430	3054306..3054620	+	315	WP_096050495.1	HigA family addiction module antidote protein	Antitoxin
CA606_RS14435	3054730..3055800	-	1071	WP_096050494.1	pseudaminic acid synthase	-
CA606_RS14440	3055924..3056184	+	261	WP_096050493.1	hypothetical protein	-
CA606_RS14445	3056174..3056401	+	228	WP_096050492.1	type II toxin-antitoxin system HicB family antitoxin	-
CA606_RS14450	3056465..3056725	-	261	WP_096050491.1	hypothetical protein	-
CA606_RS14455	3056750..3056938	-	189	WP_096050490.1	hypothetical protein	-
CA606_RS14460	3056999..3058006	+	1008	WP_096050489.1	thioredoxin-disulfide reductase	-
CA606_RS14465	3058336..3059571	-	1236	WP_096050488.1	cell wall hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (21-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-74)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10640.28 Da
• Isoelectric Point: 9.8622

>T80738 WP_096050496.1 NZ_CP023315:3053986-3054273 [Caulobacter vibrioides]
MAIQSFKDLRAKAILDGVTPGKGFPSDLIRVAKRKLEMLEAAVVLNDLRTPPGNRLEALSGHRKGQHSIRVNDQFRLVFV
WTEAGPAEVEFVDYH

Nucleotide

• Length: 288 bp

>T80738 NZ_CP023315:3053986-3054273 [Caulobacter vibrioides]
ATGGCGATCCAGAGCTTCAAGGACCTGCGCGCGAAGGCCATCCTCGACGGGGTGACGCCCGGAAAAGGTTTTCCGTCCGA
CCTCATCAGGGTCGCCAAGCGCAAGTTGGAGATGCTGGAAGCCGCCGTCGTCCTGAACGACCTCCGTACGCCACCGGGCA
ATCGGCTCGAAGCCTTGTCCGGACACCGTAAGGGTCAGCATTCGATCCGTGTCAACGATCAGTTCCGGCTGGTCTTTGTC
TGGACCGAAGCAGGACCGGCGGAGGTCGAGTTCGTGGATTATCACTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11603.48 Da
• Isoelectric Point: 7.2038

>AT80738 WP_096050495.1 NZ_CP023315:3054306-3054620 [Caulobacter vibrioides]
MRMDIAEGNDPLAPVHPGEILLEEFLKPYGLTPGRVAARLHIARPRIEKLVRGQTPVTIDTALRLERLFGASAQFWLNLQ
NRYDLEMAKRGVAPDIASIEPFAA

Nucleotide

• Length: 315 bp

>AT80738 NZ_CP023315:3054306-3054620 [Caulobacter vibrioides]
ATGCGCATGGACATCGCTGAGGGCAACGATCCCCTTGCCCCGGTCCATCCGGGCGAGATCCTGCTGGAAGAGTTCTTGAA
GCCCTATGGCCTCACGCCCGGCCGCGTCGCGGCCCGGCTTCACATCGCTCGCCCCCGGATCGAGAAGCTCGTTCGGGGTC
AAACGCCGGTGACCATCGACACCGCGCTGCGCCTGGAGCGCCTGTTCGGCGCCAGCGCGCAGTTCTGGCTCAATCTGCAG
AACCGCTACGACCTGGAAATGGCCAAGCGAGGCGTCGCGCCGGACATCGCCAGCATCGAGCCGTTCGCGGCTTAG