Detailed information of TA system TA080674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4225260..4225850
• Replicon: chromosome
• Accession: NZ_CP023256
• Organism: Alcaligenes faecalis strain c16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0A2N9E1
• Locus tag: CLH39_RS19380
• Protein ID: WP_035268575.1
• Coordinates: 4225260..4225556 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: CLH39_RS19385
• Protein ID: WP_042484646.1
• Coordinates: 4225563..4225850 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLH39_RS19365	4221047..4221940	-	894	WP_026483806.1	MurR/RpiR family transcriptional regulator	-
CLH39_RS19370	4222105..4222383	-	279	WP_009460160.1	acylphosphatase	-
CLH39_RS19375	4222561..4225125	+	2565	WP_203145207.1	EAL domain-containing protein	-
CLH39_RS19380	4225260..4225556	+	297	WP_035268575.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CLH39_RS19385	4225563..4225850	+	288	WP_042484646.1	HigA family addiction module antidote protein	Antitoxin
CLH39_RS19390	4225961..4227328	-	1368	WP_203145208.1	DNA recombination protein RmuC	-
CLH39_RS19395	4227607..4228617	+	1011	WP_042484652.1	acyltransferase family protein	-
CLH39_RS19400	4228816..4230063	-	1248	WP_083055250.1	D-amino acid dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11108.48 Da
• Isoelectric Point: 9.3459

>T80674 WP_035268575.1 NZ_CP023256:4225260-4225556 [Alcaligenes faecalis]
MIKSFRHKGLQAYFETGSKSGIQAKHAVRLQIQLTALHSATQAEDMNAPGWKLHKLSGKNPKGQSVQDHYTISVSGNWRL
TFYFEGEDAVLVDYQDYH

Nucleotide

• Length: 297 bp

>T80674 NZ_CP023256:4225260-4225556 [Alcaligenes faecalis]
ATGATTAAGAGCTTCCGACACAAGGGTCTTCAGGCTTACTTTGAAACCGGCTCCAAATCCGGCATTCAAGCCAAGCATGC
TGTTCGCCTTCAGATTCAGTTAACGGCCCTGCACTCTGCTACTCAAGCGGAAGATATGAACGCCCCCGGATGGAAGCTCC
ACAAGTTATCTGGCAAAAACCCCAAAGGCCAATCTGTACAAGATCACTACACAATATCGGTTAGCGGAAACTGGCGTCTA
ACCTTTTACTTTGAAGGCGAAGACGCTGTTTTGGTGGACTACCAGGACTATCACTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10430.96 Da
• Isoelectric Point: 9.8929

>AT80674 WP_042484646.1 NZ_CP023256:4225563-4225850 [Alcaligenes faecalis]
IMRMHNPSHPGPVLREYLGELSVTEAASRLGVTRAALSRILNGSAGISADMALRLRDALGTSAEMWINMQSHYDLWQAEQ
KPRPKVVSFHGPAHA

Nucleotide

• Length: 288 bp

>AT80674 NZ_CP023256:4225563-4225850 [Alcaligenes faecalis]
ATTATGCGTATGCACAACCCATCCCACCCCGGCCCAGTCTTGCGGGAATACTTGGGCGAACTCAGTGTCACTGAGGCGGC
ATCCCGCCTTGGCGTAACGCGTGCAGCCTTATCGCGCATTCTCAATGGTTCTGCCGGTATTTCGGCAGACATGGCCTTAC
GCCTGCGCGACGCCCTTGGAACCAGTGCCGAAATGTGGATCAACATGCAGTCCCACTACGATCTTTGGCAGGCAGAGCAA
AAGCCAAGGCCCAAAGTCGTGTCCTTTCATGGACCTGCTCATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0A2N9E1