Detailed information of TA system TA080668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 3697604..3698199
• Replicon: chromosome
• Accession: NZ_CP023255
• Organism: Pseudomonas aeruginosa strain CCUG 70744

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: CLH63_RS18030
• Protein ID: WP_003117425.1
• Coordinates: 3697921..3698199 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CLH63_RS18025
• Protein ID: WP_003099268.1
• Coordinates: 3697604..3697909 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLH63_RS18000	3693549..3693839	-	291	WP_031640334.1	DUF5447 family protein	-
CLH63_RS18005	3694015..3694287	-	273	WP_004352675.1	hypothetical protein	-
CLH63_RS18010	3694397..3694663	+	267	WP_023088595.1	hypothetical protein	-
CLH63_RS18015	3694719..3695054	+	336	WP_031637200.1	hypothetical protein	-
CLH63_RS18020	3695207..3696424	+	1218	WP_071534385.1	AAA family ATPase	-
CLH63_RS33245	3696425..3697153	+	729	WP_124075417.1	hypothetical protein	-
CLH63_RS18025	3697604..3697909	-	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
CLH63_RS18030	3697921..3698199	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CLH63_RS18040	3698528..3700756	+	2229	WP_003099265.1	TonB-dependent receptor	-
CLH63_RS18045	3700826..3701473	-	648	WP_003095021.1	carbonate dehydratase	-
CLH63_RS18050	3701535..3702773	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T80668 WP_003117425.1 NZ_CP023255:c3698199-3697921 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T80668 NZ_CP023255:c3698199-3697921 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT80668 WP_003099268.1 NZ_CP023255:c3697909-3697604 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT80668 NZ_CP023255:c3697909-3697604 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5