Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2678219..2678869 Replicon chromosome
Accession NZ_CP023185
Organism Klebsiella michiganensis strain K518

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag CKQ55_RS13260 Protein ID WP_014227314.1
Coordinates 2678219..2678560 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag CKQ55_RS13265 Protein ID WP_014227313.1
Coordinates 2678570..2678869 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKQ55_RS13245 2674482..2675801 + 1320 WP_014227316.1 MFS transporter -
CKQ55_RS13250 2675947..2677338 + 1392 WP_004107098.1 hexose-6-phosphate:phosphate antiporter -
CKQ55_RS13255 2677516..2677968 + 453 WP_014839842.1 DUF1198 domain-containing protein -
CKQ55_RS13260 2678219..2678560 + 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CKQ55_RS13265 2678570..2678869 + 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
CKQ55_RS13270 2678988..2680168 + 1181 Protein_2480 purine ribonucleoside efflux pump NepI -
CKQ55_RS13275 2680201..2681010 - 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
CKQ55_RS13280 2681020..2682123 - 1104 WP_042934427.1 2-aminoethylphosphonate--pyruvate transaminase -
CKQ55_RS13285 2682264..2682983 + 720 WP_014839839.1 phosphonate utilization transcriptional regulator PhnR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T80593 WP_014227314.1 NZ_CP023185:2678219-2678560 [Klebsiella michiganensis]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T80593 NZ_CP023185:2678219-2678560 [Klebsiella michiganensis]
ATGTGGGATGTTGAAACAACGGAGGTTTTTGACAAGTGGTTTGAGGCGCAGACCGAAGCGCTCAAAGAGGATATGCTCGC
CGCAATGGTGATCTTATCCGAGTATGGCCCCCGGTTGGGAAGGCCGTTTGCGGATACAGTAAACGATTCTGCCTTTTCGA
ATATGAAAGAGCTGCGCATTCAGCATCAAGGTAGACCCATCCGAGCTTTCTTTGTATTTGACCCTTCTCGTTGTGGCATC
GTGTTATGCGCCGGAGATAAAACAGGGTTAAATGAAAAGAGATTCTATAAAGATATGATCAAGCTTGCAGATGCTGAGTA
CCGTAAGCACCTTAATCAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT80593 WP_014227313.1 NZ_CP023185:2678570-2678869 [Klebsiella michiganensis]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT80593 NZ_CP023185:2678570-2678869 [Klebsiella michiganensis]
ATGGCGACCCTTAAAGAATTAATGGCCAAACAGAGCCCGGAAAGCCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCG
CCAGGCTATCGCTTTAAGTATGTTAAGGGAAGAACTCAATATGTCTCAGGCAGAGCTGGCTAAGGCAATGGGCGTTAAAC
AACCCACCATAGCAAAAATGGAACAGGCTGATAACGACCCGCGTTTGTCAACGCTGAAGCGCTATGTTGCAGCGCTCGGG
GGGGAACTCAGTATTGATGTTAAGCTGCCAACCGGTAAGCGAATCGCATTCCATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References