Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2418175..2418677 Replicon chromosome
Accession NZ_CP023165
Organism Escherichia coli O22:H8 strain RM10809-3

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CLC88_RS12740 Protein ID WP_000767829.1
Coordinates 2418175..2418429 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CLC88_RS12745 Protein ID WP_001259255.1
Coordinates 2418426..2418677 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLC88_RS12710 2413191..2413418 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CLC88_RS12715 2413432..2414490 - 1059 WP_000492346.1 transport protein YeeE -
CLC88_RS12720 2414669..2416027 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CLC88_RS12725 2416017..2416079 - 63 WP_010723108.1 membrane protein YoeI -
CLC88_RS12730 2416293..2417222 - 930 WP_000803351.1 LysR family transcriptional regulator -
CLC88_RS12735 2417268..2418092 - 825 WP_155705001.1 SDR family oxidoreductase -
CLC88_RS12740 2418175..2418429 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CLC88_RS12745 2418426..2418677 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CLC88_RS12750 2418960..2419010 + 51 WP_001364200.1 his operon leader peptide -
CLC88_RS12755 2419156..2420055 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CLC88_RS12760 2420061..2421365 + 1305 WP_050864003.1 histidinol dehydrogenase -
CLC88_RS12765 2421362..2422432 + 1071 WP_050864004.1 histidinol-phosphate transaminase -
CLC88_RS12770 2422432..2423499 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T80563 WP_000767829.1 NZ_CP023165:c2418429-2418175 [Escherichia coli O22:H8]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T80563 NZ_CP023165:c2418429-2418175 [Escherichia coli O22:H8]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT80563 WP_001259255.1 NZ_CP023165:c2418677-2418426 [Escherichia coli O22:H8]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT80563 NZ_CP023165:c2418677-2418426 [Escherichia coli O22:H8]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References