Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 285650..286191 Replicon chromosome
Accession NZ_CP023165
Organism Escherichia coli O22:H8 strain RM10809-3

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag CLC88_RS01920 Protein ID WP_000615982.1
Coordinates 285650..285928 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CLC88_RS01925 Protein ID WP_000729704.1
Coordinates 285931..286191 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLC88_RS01905 283232..283810 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
CLC88_RS01910 284016..284783 + 768 WP_000333380.1 class II glutamine amidotransferase -
CLC88_RS01915 284754..285494 - 741 WP_001225679.1 murein L,D-transpeptidase -
CLC88_RS01920 285650..285928 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CLC88_RS01925 285931..286191 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CLC88_RS01930 286377..287150 + 774 WP_000543899.1 C40 family peptidase -
CLC88_RS01935 287207..287563 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
CLC88_RS01940 287556..287834 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CLC88_RS01945 287939..290032 - 2094 Protein_253 flagellar biosynthesis protein FlhA -
CLC88_RS01950 290016..291155 - 1140 WP_032216948.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T80548 WP_000615982.1 NZ_CP023165:c285928-285650 [Escherichia coli O22:H8]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T80548 NZ_CP023165:c285928-285650 [Escherichia coli O22:H8]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT80548 WP_000729704.1 NZ_CP023165:c286191-285931 [Escherichia coli O22:H8]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT80548 NZ_CP023165:c286191-285931 [Escherichia coli O22:H8]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References