Detailed information of TA system TA080543

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85688..86096
• Replicon: plasmid pRM10809-3
• Accession: NZ_CP023164
• Organism: Escherichia coli O22:H8 strain RM10809-3

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: CLC88_RS00410
• Protein ID: WP_000775238.1
• Coordinates: 85688..85849 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	85907..86096 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLC88_RS00380	81560..81931	-	372	Protein_76	relaxosome protein TraM	-
CLC88_RS00385	82248..82850	+	603	WP_077698133.1	transglycosylase SLT domain-containing protein	-
CLC88_RS25580	83146..83957	-	812	Protein_78	DUF945 domain-containing protein	-
CLC88_RS25585	84071..84342	-	272	Protein_79	hypothetical protein	-
CLC88_RS00400	84511..84812	-	302	Protein_80	hypothetical protein	-
CLC88_RS25590	85105..85394	+	290	Protein_81	hypothetical protein	-
CLC88_RS00410	85688..85849	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	85907..86096	+	190	-	-	Antitoxin
-	85907..86099	-	193	NuclAT_0	-	-
-	85907..86099	-	193	NuclAT_0	-	-
-	85907..86099	-	193	NuclAT_0	-	-
-	85907..86099	-	193	NuclAT_0	-	-
CLC88_RS00415	86048..86361	-	314	Protein_83	theronine dehydrogenase	-
CLC88_RS00420	86303..87068	-	766	Protein_84	plasmid SOS inhibition protein A	-
CLC88_RS00425	87070..87496	-	427	Protein_85	conjugation system SOS inhibitor PsiB	-
CLC88_RS00430	87551..89509	-	1959	WP_050864290.1	ParB/RepB/Spo0J family partition protein	-
CLC88_RS00435	89573..89806	-	234	WP_000005988.1	DUF905 family protein	-
CLC88_RS00440	89864..90384	-	521	Protein_88	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / espP / hlyD / hlyB / hlyA / hlyC	1..122641	122641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T80543 WP_000775238.1 NZ_CP023164:c85849-85688 [Escherichia coli O22:H8]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T80543 NZ_CP023164:c85849-85688 [Escherichia coli O22:H8]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 190 bp

>AT80543 NZ_CP023164:85907-86096 [Escherichia coli O22:H8]
GCCTCGTGGGTTAATGAAAATTAACTACGGGGCTTTTGTCTTCTGCCACACAACACGGTAACAAACCACCTTCACGTCAT
GAGGCAGAAAGCCTCAAGCGCCGGGCACATCATAGCCCATATACCTGCACGCTGACCACACTCACTTTCCCTGAAAATAA
TCCGGTCGTTCAGCCAGTTCACGGGCAATC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin