Detailed information of TA system TA080541

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44044..44298
• Replicon: plasmid pRM10809-3
• Accession: NZ_CP023164
• Organism: Escherichia coli O22:H8 strain RM10809-3

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CLC88_RS00170
• Protein ID: WP_032186826.1
• Coordinates: 44044..44250 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	44237..44298 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLC88_RS00150	41128..41367	-	240	WP_000859018.1	MarR family transcriptional regulator	-
CLC88_RS00155	42343..43200	-	858	WP_028985401.1	incFII family plasmid replication initiator RepA	-
CLC88_RS00160	43193..43267	-	75	WP_062865987.1	RepA leader peptide Tap	-
CLC88_RS00165	43503..43760	-	258	WP_000084404.1	replication regulatory protein RepA	-
CLC88_RS00170	44044..44250	-	207	WP_032186826.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	44237..44298	+	62	NuclAT_1	-	Antitoxin
-	44237..44298	+	62	NuclAT_1	-	Antitoxin
-	44237..44298	+	62	NuclAT_1	-	Antitoxin
-	44237..44298	+	62	NuclAT_1	-	Antitoxin
CLC88_RS25575	44473..44856	-	384	Protein_36	hypothetical protein	-
CLC88_RS00180	44959..45418	-	460	Protein_37	thermonuclease family protein	-
CLC88_RS00185	46161..46364	-	204	WP_032295436.1	hypothetical protein	-
CLC88_RS00190	46519..47076	-	558	WP_028985402.1	fertility inhibition protein FinO	-
CLC88_RS00195	47131..47877	-	747	WP_044804172.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / espP / hlyD / hlyB / hlyA / hlyC	1..122641	122641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7822.32 Da
• Isoelectric Point: 8.8807

>T80541 WP_032186826.1 NZ_CP023164:c44250-44044 [Escherichia coli O22:H8]
MKYLNTTDCSLFLAERSKFMTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T80541 NZ_CP023164:c44250-44044 [Escherichia coli O22:H8]
ATGAAGTATCTCAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATACCCTAATTGG
GTTGCTCGCTGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT80541 NZ_CP023164:44237-44298 [Escherichia coli O22:H8]
TTGAGATACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin