Detailed information of TA system TA080495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4042369..4043019
• Replicon: chromosome
• Accession: NZ_CP023142
• Organism: Escherichia coli strain CFSAN061770

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CA696_RS21115
• Protein ID: WP_000263532.1
• Coordinates: 4042678..4043019 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: U9YXQ7
• Locus tag: CA696_RS21110
• Protein ID: WP_000212552.1
• Coordinates: 4042369..4042668 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA696_RS21080	4037817..4038128	-	312	WP_000415004.1	hypothetical protein	-
CA696_RS21085	4038133..4038525	-	393	WP_000285322.1	flagellar export chaperone FliS	-
CA696_RS21090	4038548..4039864	-	1317	WP_000609678.1	flagellar filament capping protein FliD	-
CA696_RS21100	4040069..4040983	-	915	WP_000949083.1	lateral flagellin LafA	-
CA696_RS21105	4041469..4042311	+	843	WP_000022366.1	winged helix-turn-helix domain-containing protein	-
CA696_RS21110	4042369..4042668	-	300	WP_000212552.1	helix-turn-helix transcriptional regulator	Antitoxin
CA696_RS21115	4042678..4043019	-	342	WP_000263532.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CA696_RS21120	4043095..4044072	-	978	WP_001195938.1	hypothetical protein	-
CA696_RS21125	4044089..4045018	-	930	WP_001266799.1	flagellar hook-associated protein FlgL	-
CA696_RS21130	4045033..4046409	-	1377	WP_000367245.1	flagellar hook-associated protein FlgK	-
CA696_RS21135	4046585..4046884	-	300	WP_000867281.1	rod-binding protein	-
CA696_RS21140	4046884..4047984	-	1101	WP_001181876.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13031.86 Da
• Isoelectric Point: 6.4677

>T80495 WP_000263532.1 NZ_CP023142:c4043019-4042678 [Escherichia coli]
MWDVETTETFDNWFDAQTVALKEDLLAAMLILAEYGPQLGRPFADTVNDSQFSNMKELRVQHQGNPVRAFFAFDPARRGI
VLCAGDKTGLNEKKFYRDMIKLADSEYRKHLKK

Nucleotide

• Length: 342 bp

>T80495 NZ_CP023142:c4043019-4042678 [Escherichia coli]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTACTTGC
TGCAATGCTGATTCTTGCTGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGATACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCGGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11004.86 Da
• Isoelectric Point: 9.7665

>AT80495 WP_000212552.1 NZ_CP023142:c4042668-4042369 [Escherichia coli]
MATLKELMAKQSPESQVRIAAKVEEMRQTIALHQLREELNISQTEMAAAMGIKQPTLAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT80495 NZ_CP023142:c4042668-4042369 [Escherichia coli]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGTGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACTCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTTGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCGAATGGTAAGAGAATTGCATTTCAGATTTAA

Structures

Antitoxin

• AlphaFold DB: U9YXQ7