Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2597034..2597596 Replicon chromosome
Accession NZ_CP023142
Organism Escherichia coli strain CFSAN061770

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag CA696_RS13260 Protein ID WP_000605676.1
Coordinates 2597034..2597312 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag CA696_RS13265 Protein ID WP_000781370.1
Coordinates 2597312..2597596 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CA696_RS13230 2592620..2593051 - 432 WP_000152305.1 peroxiredoxin OsmC -
CA696_RS13240 2593396..2593611 + 216 WP_000495766.1 biofilm-dependent modulation protein -
CA696_RS13245 2593713..2593850 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
CA696_RS13250 2594007..2595704 + 1698 WP_000433464.1 malate dehydrogenase -
CA696_RS13255 2595838..2596848 + 1011 WP_095454374.1 alcohol dehydrogenase AdhP -
CA696_RS13260 2597034..2597312 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CA696_RS13265 2597312..2597596 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
CA696_RS13270 2597824..2598477 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
CA696_RS13275 2598470..2599354 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
CA696_RS13280 2599367..2602414 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T80490 WP_000605676.1 NZ_CP023142:2597034-2597312 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T80490 NZ_CP023142:2597034-2597312 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT80490 WP_000781370.1 NZ_CP023142:2597312-2597596 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT80490 NZ_CP023142:2597312-2597596 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References