Detailed information of TA system TA080461

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 860547..860961
• Replicon: chromosome
• Accession: NZ_CP023076
• Organism: Staphylococcus argenteus strain XNO62

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CJ017_RS04230
• Protein ID: WP_100848567.1
• Coordinates: 860547..860798 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CJ017_RS13510
• Protein ID: WP_000028424.1
• Coordinates: 860788..860961 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJ017_RS04180	855891..856565	+	675	WP_088815076.1	hypothetical protein	-
CJ017_RS04185	856675..857334	-	660	WP_016028252.1	hypothetical protein	-
CJ017_RS04190	857380..858150	+	771	WP_000190226.1	conserved phage C-terminal domain-containing protein	-
CJ017_RS04195	858160..858933	+	774	WP_070002564.1	ATP-binding protein	-
CJ017_RS04200	858927..859085	+	159	WP_000256594.1	hypothetical protein	-
CJ017_RS04205	859098..859319	+	222	WP_001123695.1	DUF3269 family protein	-
CJ017_RS04210	859329..859733	+	405	WP_088808346.1	DUF1064 domain-containing protein	-
CJ017_RS04215	859738..859926	+	189	WP_088808347.1	DUF3113 family protein	-
CJ017_RS04220	859927..860286	+	360	WP_031824891.1	phage protein	-
CJ017_RS04225	860290..860532	+	243	WP_100848566.1	phi PVL orf 51-like protein	-
CJ017_RS04230	860547..860798	+	252	WP_100848567.1	DUF1024 family protein	Toxin
CJ017_RS13510	860788..860961	+	174	WP_000028424.1	hypothetical protein	Antitoxin
CJ017_RS04235	860962..861243	+	282	WP_000454994.1	hypothetical protein	-
CJ017_RS13515	861244..861405	+	162	WP_000889683.1	hypothetical protein	-
CJ017_RS04240	861420..861947	+	528	WP_072473165.1	dUTPase	-
CJ017_RS04245	861984..862190	+	207	WP_029625633.1	DUF1381 domain-containing protein	-
CJ017_RS04250	862187..862360	+	174	WP_029625632.1	transcriptional activator RinB	-
CJ017_RS04255	862361..862507	+	147	WP_000990005.1	hypothetical protein	-
CJ017_RS04260	862522..862941	+	420	WP_000058632.1	transcriptional regulator	-
CJ017_RS04265	863128..863568	+	441	WP_031883675.1	terminase small subunit	-
CJ017_RS04270	863555..864832	+	1278	WP_119875091.1	PBSX family phage terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	vWbp	812378..906748	94370

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9460.36 Da
• Isoelectric Point: 3.9373

>T80461 WP_100848567.1 NZ_CP023076:860547-860798 [Staphylococcus argenteus]
MNNREQIEQSIISASAYNGNDTEGLLKEVEDVYKKAQAFDEILDGMTNAIQHSVKEGVELDEAVRIMAGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T80461 NZ_CP023076:860547-860798 [Staphylococcus argenteus]
ATGAATAACCGCGAACAAATTGAACAATCAATTATCAGTGCTAGTGCCTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGGTTGAAGACGTGTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGATGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAAGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT80461 WP_000028424.1 NZ_CP023076:860788-860961 [Staphylococcus argenteus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT80461 NZ_CP023076:860788-860961 [Staphylococcus argenteus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCCGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTGA