Detailed information of TA system TA080434

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 11572..11842
• Replicon: plasmid pFORC69
• Accession: NZ_CP023062
• Organism: Escherichia coli strain FORC_069

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: FORC69_RS27265
• Protein ID: WP_001388592.1
• Coordinates: 11684..11842 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	11572..11635 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FORC69_RS27225	7514..7696	+	183	Protein_9	class I SAM-dependent methyltransferase	-
FORC69_RS28285	7782..7898	-	117	Protein_10	pilus protein	-
FORC69_RS27235	8035..9702	-	1668	WP_001091724.1	group II intron reverse transcriptase/maturase	-
FORC69_RS27255	10802..11404	+	603	Protein_12	plasmid SOS inhibition protein A	-
-	11416..11640	+	225	NuclAT_0	-	-
-	11416..11640	+	225	NuclAT_0	-	-
-	11416..11640	+	225	NuclAT_0	-	-
-	11416..11640	+	225	NuclAT_0	-	-
FORC69_RS27260	11425..11604	-	180	WP_001309233.1	hypothetical protein	-
-	11572..11635	-	64	-	-	Antitoxin
FORC69_RS27265	11684..11842	+	159	WP_001388592.1	type I toxin-antitoxin system Hok family toxin	Toxin
FORC69_RS28115	12080..12645	-	566	Protein_15	hypothetical protein	-
FORC69_RS27280	12824..13645	+	822	WP_001234482.1	DUF945 domain-containing protein	-
FORC69_RS27290	13940..14530	-	591	WP_176453891.1	transglycosylase SLT domain-containing protein	-
FORC69_RS27295	14870..15253	+	384	WP_001151566.1	relaxosome protein TraM	-
FORC69_RS27300	15447..16139	+	693	WP_000332477.1	PAS domain-containing protein	-
FORC69_RS27305	16227..16454	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaTEM-1B	agg3D / agg3C / agg3B / agg3A / aap/aspU / pic	1..77306	77306

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6135.36 Da
• Isoelectric Point: 10.1101

>T80434 WP_001388592.1 NZ_CP023062:11684-11842 [Escherichia coli]
MKLPRSSLVWRVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T80434 NZ_CP023062:11684-11842 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGCGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT80434 NZ_CP023062:c11635-11572 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Antitoxin