Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4099961..4100502 Replicon chromosome
Accession NZ_CP023061
Organism Escherichia coli strain FORC_069

Toxin (Protein)


Gene name yafQ Uniprot ID B7LHF6
Locus tag FORC69_RS21720 Protein ID WP_000615974.1
Coordinates 4100224..4100502 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FORC69_RS21715 Protein ID WP_000729704.1
Coordinates 4099961..4100221 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC69_RS21690 4095744..4096529 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
FORC69_RS21695 4096501..4098213 + 1713 Protein_3955 flagellar biosynthesis protein FlhA -
FORC69_RS21700 4098318..4098596 + 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
FORC69_RS21705 4098589..4098945 + 357 WP_001030479.1 type II toxin-antitoxin system HicB family antitoxin -
FORC69_RS21710 4099002..4099775 - 774 WP_000543895.1 C40 family peptidase -
FORC69_RS21715 4099961..4100221 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FORC69_RS21720 4100224..4100502 + 279 WP_000615974.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FORC69_RS21725 4100658..4101398 + 741 WP_001225679.1 murein L,D-transpeptidase -
FORC69_RS21730 4101369..4102136 - 768 WP_000333380.1 class II glutamine amidotransferase -
FORC69_RS21735 4102342..4102920 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10855.68 Da        Isoelectric Point: 10.2267

>T80429 WP_000615974.1 NZ_CP023061:4100224-4100502 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMRLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T80429 NZ_CP023061:4100224-4100502 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGAGGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT80429 WP_000729704.1 NZ_CP023061:4099961-4100221 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT80429 NZ_CP023061:4099961-4100221 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y514

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References