Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1895755..1896257 Replicon chromosome
Accession NZ_CP023061
Organism Escherichia coli strain FORC_069

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FORC69_RS09640 Protein ID WP_000767829.1
Coordinates 1896003..1896257 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FORC69_RS09635 Protein ID WP_001259255.1
Coordinates 1895755..1896006 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC69_RS09605 1890932..1891999 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FORC69_RS09610 1891999..1893069 - 1071 WP_000108967.1 histidinol-phosphate transaminase -
FORC69_RS09615 1893066..1894370 - 1305 WP_001386882.1 histidinol dehydrogenase -
FORC69_RS09620 1894376..1895275 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FORC69_RS09630 1895421..1895471 - 51 WP_001364200.1 his operon leader peptide -
FORC69_RS09635 1895755..1896006 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FORC69_RS09640 1896003..1896257 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FORC69_RS09645 1896340..1897164 + 825 WP_000754758.1 SDR family oxidoreductase -
FORC69_RS09650 1897210..1898139 + 930 WP_000803366.1 LysR family transcriptional regulator -
FORC69_RS09655 1898354..1898416 + 63 WP_010723108.1 membrane protein YoeI -
FORC69_RS09660 1898406..1899764 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FORC69_RS09665 1899943..1901001 + 1059 WP_000492324.1 transport protein YeeE -
FORC69_RS09670 1901015..1901242 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T80415 WP_000767829.1 NZ_CP023061:1896003-1896257 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T80415 NZ_CP023061:1896003-1896257 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT80415 WP_001259255.1 NZ_CP023061:1895755-1896006 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT80415 NZ_CP023061:1895755-1896006 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References