Detailed information of TA system TA080401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 453414..454029
• Replicon: chromosome
• Accession: NZ_CP023055
• Organism: Vibrio anguillarum strain VIB43

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CK207_RS17310
• Protein ID: WP_029388214.1
• Coordinates: 453414..453701 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CK207_RS17315
• Protein ID: WP_001232701.1
• Coordinates: 453712..454029 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CK207_RS17265	449179..449658	+	480	WP_017045717.1	lecithin retinol acyltransferase family protein	-
CK207_RS17275	449800..450078	-	279	WP_010320663.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
CK207_RS17280	450075..450359	-	285	WP_017040620.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
CK207_RS17285	450557..451062	+	506	Protein_408	lipocalin family protein	-
CK207_RS17295	452518..452760	+	243	WP_095661368.1	DUF1289 domain-containing protein	-
CK207_RS17300	452880..453110	-	231	WP_013868597.1	PAS factor family protein	-
CK207_RS17310	453414..453701	+	288	WP_029388214.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CK207_RS17315	453712..454029	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
CK207_RS17325	454178..454594	+	417	WP_017045395.1	VOC family protein	-
CK207_RS17330	454607..455578	-	972	WP_019282910.1	IS110 family transposase	-
CK207_RS17340	456011..456331	+	321	WP_019283194.1	IS66 family insertion sequence hypothetical protein	-
CK207_RS17345	456328..456681	+	354	WP_019283195.1	IS66 family insertion sequence element accessory protein TnpB	-
CK207_RS17350	456741..458273	+	1533	WP_069211897.1	IS66-like element ISVa11 family transposase	-
CK207_RS17355	458535..458888	-	354	Protein_419	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	451369..475643	24274
	-	inside	Integron	-	-	446572..456331	9759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11350.93 Da
• Isoelectric Point: 6.7187

>T80401 WP_029388214.1 NZ_CP023055:453414-453701 [Vibrio anguillarum]
MALEFKDKWLEQFYEDDKRHRLIPSSIESALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHRY

Nucleotide

• Length: 288 bp

>T80401 NZ_CP023055:453414-453701 [Vibrio anguillarum]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAGTGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCGCCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAGGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT80401 WP_001232701.1 NZ_CP023055:453712-454029 [Vibrio anguillarum]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT80401 NZ_CP023055:453712-454029 [Vibrio anguillarum]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTGAGTAATTTGATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCAGCGGCATTAGGCAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA