Detailed information of TA system TA080294

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2088071..2088824
• Replicon: chromosome
• Accession: NZ_CP022989
• Organism: Paraburkholderia aromaticivorans strain BN5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: CJU94_RS09615
• Protein ID: WP_095418490.1
• Coordinates: 2088543..2088824 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CJU94_RS09610
• Protein ID: WP_095418489.1
• Coordinates: 2088071..2088526 (-)
• Length: 152 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJU94_RS09585	2083220..2083465	-	246	WP_095418485.1	hypothetical protein	-
CJU94_RS09590	2083527..2086250	-	2724	WP_095418486.1	excinuclease ABC subunit UvrA	-
CJU94_RS09595	2086491..2086691	+	201	WP_095418487.1	hypothetical protein	-
CJU94_RS09605	2086932..2087948	-	1017	WP_095418488.1	class 1 fructose-bisphosphatase	-
CJU94_RS09610	2088071..2088526	-	456	WP_095418489.1	HigA family addiction module antidote protein	Antitoxin
CJU94_RS09615	2088543..2088824	-	282	WP_095418490.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CJU94_RS09620	2089020..2091716	-	2697	WP_095418491.1	aminopeptidase N	-
CJU94_RS09625	2091946..2092644	+	699	WP_095418492.1	DUF4136 domain-containing protein	-
CJU94_RS09630	2093052..2093624	-	573	WP_095418493.1	TMEM165/GDT1 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (22-115)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10564.13 Da
• Isoelectric Point: 9.1121

>T80294 WP_095418490.1 NZ_CP022989:c2088824-2088543 [Paraburkholderia aromaticivorans]
MIATFGDKATASIFQGRFVYSLPLHMPTLARRKLLMIDSAESVRDLHAPPGNRLEAPQGQRSGQWSIRINAQWRICFHFV
DGQALNVEIVDYH

Nucleotide

• Length: 282 bp

>T80294 NZ_CP022989:c2088824-2088543 [Paraburkholderia aromaticivorans]
ATGATCGCGACATTCGGCGACAAAGCTACGGCGAGCATTTTTCAAGGCAGGTTCGTGTATTCATTGCCGCTTCACATGCC
GACGCTCGCGCGCCGCAAGTTGCTGATGATCGACTCCGCAGAATCCGTCCGCGACCTGCACGCGCCACCCGGCAACCGGC
TGGAAGCACCGCAGGGCCAACGCAGCGGGCAGTGGAGTATCCGGATCAATGCGCAATGGCGCATCTGTTTTCATTTCGTC
GACGGGCAGGCGCTGAATGTCGAAATTGTCGACTATCACTGA

Antitoxin

Protein

• Length: 152 a.a.
• Molecular weight: 16491.97 Da
• Isoelectric Point: 9.6812

>AT80294 WP_095418489.1 NZ_CP022989:c2088526-2088071 [Paraburkholderia aromaticivorans]
MVIKRSDLDSIDFSDIDTGNSIPEIHPGEILRSEFLEPLGMSVNALALALRVPAPRINDVVRCRRAISADTALRLERYFG
ASAQFWLNLQIAYDLRVATAAAGEQIEREIEPMPKADRPKLPKVSSEHARAAALAASVSGKLTASKARRKA

Nucleotide

• Length: 456 bp

>AT80294 NZ_CP022989:c2088526-2088071 [Paraburkholderia aromaticivorans]
ATGGTTATCAAACGCTCCGATCTGGACAGCATCGATTTCTCGGACATTGACACCGGCAACAGCATCCCGGAAATTCATCC
GGGCGAGATTCTGCGCAGCGAGTTCCTCGAACCGCTCGGCATGTCCGTCAATGCACTCGCACTGGCGCTGCGCGTACCGG
CGCCGCGCATCAACGACGTCGTGCGCTGCAGGCGCGCTATTTCGGCCGACACCGCGTTGCGGCTCGAGCGCTACTTTGGC
GCGAGCGCGCAGTTCTGGCTCAACCTGCAAATCGCTTACGACCTGCGCGTCGCCACCGCGGCCGCCGGCGAACAGATCGA
ACGTGAAATCGAGCCCATGCCGAAAGCGGACCGCCCGAAACTGCCGAAGGTGTCGAGCGAACACGCCCGGGCCGCGGCGC
TCGCCGCGAGCGTGAGCGGCAAGCTCACAGCCAGCAAGGCGCGCCGCAAAGCCTGA