Detailed information of TA system TA080288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 663106..663695
• Replicon: chromosome
• Accession: NZ_CP022987
• Organism: Pusillimonas thiosulfatoxidans strain YE3

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: CKA81_RS03220
• Protein ID: WP_128354017.1
• Coordinates: 663106..663384 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CKA81_RS03225
• Protein ID: WP_128354018.1
• Coordinates: 663396..663695 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CKA81_RS03210	659567..660886	-	1320	WP_128354015.1	FliC/FljB family flagellin	-
CKA81_RS03215	661059..662711	-	1653	WP_128354016.1	PAS domain-containing methyl-accepting chemotaxis protein	-
CKA81_RS03220	663106..663384	+	279	WP_128354017.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CKA81_RS03225	663396..663695	+	300	WP_128354018.1	HigA family addiction module antidote protein	Antitoxin
CKA81_RS03230	663795..665669	-	1875	WP_128354019.1	TRAP transporter large permease subunit	-
CKA81_RS03235	665666..666292	-	627	WP_128354020.1	TRAP transporter small permease subunit	-
CKA81_RS03240	666420..667577	-	1158	WP_128354021.1	TRAP transporter substrate-binding protein	-
CKA81_RS03245	667889..668074	-	186	WP_128354022.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (23-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-90)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10603.19 Da
• Isoelectric Point: 10.3041

>T80288 WP_128354017.1 NZ_CP022987:663106-663384 [Pusillimonas thiosulfatoxidans]
MINSFACSDTEMLFNSRPVLRLANIERAARRKLLQLHAAESLDSLRIPPGNRLEALRGRRAGQDSMRINKQWRVCFKWTT
GGAYDVEIVDYH

Nucleotide

• Length: 279 bp

>T80288 NZ_CP022987:663106-663384 [Pusillimonas thiosulfatoxidans]
ATGATCAACTCATTTGCCTGCTCAGATACGGAGATGTTGTTTAACAGCCGTCCGGTGCTTCGACTAGCGAATATAGAGCG
GGCCGCAAGGCGAAAGCTTTTACAGTTGCACGCCGCAGAATCATTGGACAGCTTGCGGATTCCTCCAGGCAATCGGCTGG
AGGCTTTAAGAGGTAGGCGCGCTGGCCAAGACAGCATGCGCATTAACAAGCAGTGGCGAGTGTGTTTTAAATGGACGACC
GGCGGCGCATATGATGTTGAGATCGTCGATTATCACTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11630.52 Da
• Isoelectric Point: 8.5276

>AT80288 WP_128354018.1 NZ_CP022987:663396-663695 [Pusillimonas thiosulfatoxidans]
MDKLLDEIHPGEILREEFMRPLGITAQRLAADIDVPPSRISEILRGKRPITADTALRLGMFFNMEVRYWLNLQTEYDVRV
AYRNLEAKIRSRIRVFTPD

Nucleotide

• Length: 300 bp

>AT80288 NZ_CP022987:663396-663695 [Pusillimonas thiosulfatoxidans]
ATGGATAAATTGCTCGACGAGATCCATCCGGGCGAAATTCTACGGGAAGAATTTATGAGGCCGCTGGGCATCACTGCTCA
GCGTCTCGCGGCCGACATTGATGTGCCGCCTAGCCGCATCAGCGAAATCCTCCGTGGGAAGCGGCCCATCACGGCCGATA
CGGCATTACGCTTGGGCATGTTCTTTAATATGGAAGTGCGCTATTGGCTCAATCTTCAGACAGAATACGACGTGCGCGTG
GCCTATCGTAATTTGGAAGCCAAGATCCGTTCTCGCATCCGGGTATTTACACCCGACTGA