Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3712835..3713376 Replicon chromosome
Accession NZ_CP022959
Organism Escherichia coli ATCC 8739

Toxin (Protein)


Gene name yafQ Uniprot ID S1FHQ9
Locus tag CJZ69_RS18495 Protein ID WP_000615979.1
Coordinates 3713098..3713376 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CJZ69_RS18490 Protein ID WP_000729704.1
Coordinates 3712835..3713095 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJZ69_RS18475 3708894..3710987 + 2094 WP_001140809.1 flagellar biosynthesis protein FlhA -
CJZ69_RS18480 3711203..3711700 - 498 WP_000006261.1 REP-associated tyrosine transposase RayT -
CJZ69_RS18485 3711876..3712649 - 774 WP_001353765.1 C40 family peptidase -
CJZ69_RS18490 3712835..3713095 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CJZ69_RS18495 3713098..3713376 + 279 WP_000615979.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CJZ69_RS18500 3713532..3714272 + 741 WP_001225679.1 murein L,D-transpeptidase -
CJZ69_RS18505 3714243..3715010 - 768 WP_000333379.1 class II glutamine amidotransferase -
CJZ69_RS18510 3715216..3715794 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3711203..3731876 20673


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T80269 WP_000615979.1 NZ_CP022959:3713098-3713376 [Escherichia coli ATCC 8739]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T80269 NZ_CP022959:3713098-3713376 [Escherichia coli ATCC 8739]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT80269 WP_000729704.1 NZ_CP022959:3712835-3713095 [Escherichia coli ATCC 8739]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT80269 NZ_CP022959:3712835-3713095 [Escherichia coli ATCC 8739]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHQ9

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References