Detailed information of TA system TA080251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 613638..614437
• Replicon: chromosome
• Accession: NZ_CP022959
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: CJZ69_RS03065
• Protein ID: WP_000347251.1
• Coordinates: 613638..614102 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: CJZ69_RS03070
• Protein ID: WP_012304834.1
• Coordinates: 614102..614437 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJZ69_RS03035	608639..609073	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
CJZ69_RS03040	609091..609969	-	879	WP_012304833.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CJZ69_RS03045	609959..610738	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CJZ69_RS03050	610749..611222	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CJZ69_RS03055	611245..612525	-	1281	WP_000681905.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CJZ69_RS03060	612774..613583	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CJZ69_RS03065	613638..614102	-	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CJZ69_RS03070	614102..614437	-	336	WP_012304834.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CJZ69_RS03075	614586..616157	-	1572	WP_001273760.1	galactarate dehydratase	-
CJZ69_RS03080	616532..617866	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
CJZ69_RS03085	617882..618652	+	771	WP_001058236.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T80251 WP_000347251.1 NZ_CP022959:c614102-613638 [Escherichia coli ATCC 8739]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T80251 NZ_CP022959:c614102-613638 [Escherichia coli ATCC 8739]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12344.95 Da
• Isoelectric Point: 4.6890

>AT80251 WP_012304834.1 NZ_CP022959:c614437-614102 [Escherichia coli ATCC 8739]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT80251 NZ_CP022959:c614437-614102 [Escherichia coli ATCC 8739]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCCGGCCTGGACAGCATTCATTACGAAATTCTCCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20