Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 350240..350886 Replicon chromosome
Accession NZ_CP022919
Organism Klebsiella pneumoniae strain ST307PT03

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag CJ256_RS01675 Protein ID WP_016529833.1
Coordinates 350240..350587 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag CJ256_RS01680 Protein ID WP_002920557.1
Coordinates 350587..350886 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CJ256_RS01665 346176..347609 + 1434 WP_002920564.1 glycogen synthase GlgA -
CJ256_RS01670 347627..350074 + 2448 WP_002920561.1 glycogen phosphorylase -
CJ256_RS01675 350240..350587 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CJ256_RS01680 350587..350886 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
CJ256_RS01685 350949..352457 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
CJ256_RS01690 352662..352991 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
CJ256_RS01695 353042..353872 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
CJ256_RS01700 353922..354680 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T80181 WP_016529833.1 NZ_CP022919:350240-350587 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T80181 NZ_CP022919:350240-350587 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT80181 WP_002920557.1 NZ_CP022919:350587-350886 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT80181 NZ_CP022919:350587-350886 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References