Detailed information of TA system TA080150

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 55550..55911
• Replicon: plasmid unnamed1
• Accession: NZ_CP022913
• Organism: Escherichia coli O6:H16 strain 2011EL-1370-2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: CJZ79_RS25530
• Protein ID: WP_000775238.1
• Coordinates: 55550..55711 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	55777..55911 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJZ79_RS25495	50800..51477	+	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
CJZ79_RS25500	51477..51824	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
CJZ79_RS25505	51844..53415	+	1572	WP_000381401.1	IS66 family transposase	-
CJZ79_RS25510	53442..54248	-	807	WP_001705702.1	DUF945 domain-containing protein	-
CJZ79_RS25515	54359..54655	-	297	WP_001272251.1	hypothetical protein	-
CJZ79_RS26950	54995..55312	+	318	WP_000820288.1	hypothetical protein	-
CJZ79_RS25530	55550..55711	-	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	55777..55911	-	135	NuclAT_0	-	Antitoxin
-	55777..55911	-	135	NuclAT_0	-	Antitoxin
-	55777..55911	-	135	NuclAT_0	-	Antitoxin
-	55777..55911	-	135	NuclAT_0	-	Antitoxin
CJZ79_RS25540	55887..56405	-	519	WP_001178556.1	hypothetical protein	-
CJZ79_RS25545	56405..58081	-	1677	WP_052158500.1	plasmid SOS inhibition protein A	-
CJZ79_RS25550	58145..58378	-	234	WP_000005971.1	DUF905 domain-containing protein	-
CJZ79_RS25555	58435..58962	-	528	WP_000290820.1	single-stranded DNA-binding protein	-
CJZ79_RS26955	59264..59702	+	439	Protein_69	hypothetical protein	-
CJZ79_RS25575	59788..60351	-	564	WP_001297832.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cofA	1..63392	63392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T80150 WP_000775238.1 NZ_CP022913:c55711-55550 [Escherichia coli O6:H16]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T80150 NZ_CP022913:c55711-55550 [Escherichia coli O6:H16]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 135 bp

>AT80150 NZ_CP022913:c55911-55777 [Escherichia coli O6:H16]
CAGGGTGCAGACATATGGGCTATGATGTGCCCGGCGCTTGAGGCTTTTTGCCTCATGACGTGAAGGTGGTTTGTTACCGT
GTTGTGTGGCAGAAAGCAGAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin