Detailed information of TA system TA080148

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 44162..44416
• Replicon: plasmid unnamed1
• Accession: NZ_CP022913
• Organism: Escherichia coli O6:H16 strain 2011EL-1370-2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CJZ79_RS25430
• Protein ID: WP_032186826.1
• Coordinates: 44162..44368 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	44360..44416 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJZ79_RS25380	39412..40593	-	1182	WP_001467544.1	DUF3800 domain-containing protein	-
CJZ79_RS25390	40851..41438	+	588	Protein_43	IS91 family transposase	-
CJZ79_RS26945	41589..41768	-	180	WP_032231585.1	hypothetical protein	-
CJZ79_RS25410	42471..43328	-	858	WP_000130964.1	incFII family plasmid replication initiator RepA	-
CJZ79_RS25415	43321..43395	-	75	WP_001365571.1	RepA leader peptide Tap	-
CJZ79_RS25425	43618..43878	-	261	WP_000083817.1	replication regulatory protein RepA	-
CJZ79_RS25430	44162..44368	-	207	WP_032186826.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	44360..44416	+	57	NuclAT_1	-	Antitoxin
-	44360..44416	+	57	NuclAT_1	-	Antitoxin
-	44360..44416	+	57	NuclAT_1	-	Antitoxin
-	44360..44416	+	57	NuclAT_1	-	Antitoxin
CJZ79_RS25440	44660..44842	+	183	WP_001705320.1	hypothetical protein	-
CJZ79_RS25445	45067..45480	-	414	WP_077625677.1	type-F conjugative transfer system pilin acetylase TraX	-
CJZ79_RS25450	46143..47594	-	1452	Protein_51	type IV conjugative transfer system coupling protein TraD	-
CJZ79_RS25455	47594..47845	-	252	Protein_52	conjugal transfer protein TraK	-
CJZ79_RS25460	47835..48398	-	564	WP_000406020.1	type IV conjugative transfer system protein TraE	-
CJZ79_RS25465	48418..48723	-	306	WP_000016323.1	type IV conjugative transfer system protein TraL	-
CJZ79_RS25470	48725..49084	-	360	WP_001054220.1	type IV conjugative transfer system pilin TraA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cofA	1..63392	63392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7822.32 Da
• Isoelectric Point: 8.8807

>T80148 WP_032186826.1 NZ_CP022913:c44368-44162 [Escherichia coli O6:H16]
MKYLNTTDCSLFLAERSKFMTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T80148 NZ_CP022913:c44368-44162 [Escherichia coli O6:H16]
ATGAAGTACTTGAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATACCCTTATTGG
GTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTTTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT80148 NZ_CP022913:44360-44416 [Escherichia coli O6:H16]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin