Detailed information of TA system TA080146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3758617..3759311
• Replicon: chromosome
• Accession: NZ_CP022912
• Organism: Escherichia coli O6:H16 strain 2011EL-1370-2

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: CJZ79_RS19870
• Protein ID: WP_001263493.1
• Coordinates: 3758617..3759015 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CJZ79_RS19875
• Protein ID: WP_000554757.1
• Coordinates: 3759018..3759311 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJZ79_RS19840	3753617..3754861	-	1245	WP_000189541.1	esterase FrsA	-
-	3754277..3754357	-	81	NuclAT_12	-	-
-	3754277..3754357	-	81	NuclAT_12	-	-
-	3754277..3754357	-	81	NuclAT_12	-	-
-	3754277..3754357	-	81	NuclAT_12	-	-
CJZ79_RS19845	3754953..3755411	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CJZ79_RS19850	3755672..3757129	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
CJZ79_RS19855	3757186..3757707	-	522	Protein_3618	peptide chain release factor H	-
CJZ79_RS19860	3757706..3757909	-	204	Protein_3619	RNA ligase RtcB family protein	-
CJZ79_RS19865	3758155..3758607	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
CJZ79_RS19870	3758617..3759015	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CJZ79_RS19875	3759018..3759311	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CJZ79_RS19880	3759363..3760418	-	1056	WP_001226164.1	DNA polymerase IV	-
CJZ79_RS19885	3760489..3761412	-	924	WP_001232570.1	putative lateral flagellar export/assembly protein LafU	-
CJZ79_RS19890	3761415..3762278	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
CJZ79_RS19895	3762291..3763010	-	720	WP_000938740.1	FliA/WhiG family RNA polymerase sigma factor	-
CJZ79_RS19900	3763030..3763497	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T80146 WP_001263493.1 NZ_CP022912:c3759015-3758617 [Escherichia coli O6:H16]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T80146 NZ_CP022912:c3759015-3758617 [Escherichia coli O6:H16]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT80146 WP_000554757.1 NZ_CP022912:c3759311-3759018 [Escherichia coli O6:H16]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT80146 NZ_CP022912:c3759311-3759018 [Escherichia coli O6:H16]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6