Detailed information of TA system TA080069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2552102..2552619
• Replicon: chromosome
• Accession: NZ_CP022905
• Organism: Staphylococcus aureus strain 628

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: CGQ03_RS13435
• Protein ID: WP_000113265.1
• Coordinates: 2552102..2552368 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: CGQ03_RS13440
• Protein ID: WP_000584499.1
• Coordinates: 2552368..2552619 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CGQ03_RS13405	2547530..2548066	-	537	WP_000169317.1	GNAT family N-acetyltransferase	-
CGQ03_RS13410	2548300..2549124	-	825	WP_000572038.1	formate/nitrite transporter family protein	-
CGQ03_RS13415	2549341..2549523	-	183	WP_000230292.1	hypothetical protein	-
CGQ03_RS13420	2549607..2550074	-	468	WP_000153347.1	SRPBCC domain-containing protein	-
CGQ03_RS13425	2550260..2551807	-	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
CGQ03_RS13430	2551996..2552067	-	72	Protein_2480	hypothetical protein	-
CGQ03_RS13435	2552102..2552368	-	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
CGQ03_RS13440	2552368..2552619	-	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CGQ03_RS13445	2552784..2552885	-	102	Protein_2483	hypothetical protein	-
CGQ03_RS13450	2552892..2553491	-	600	WP_000162810.1	DsbA family protein	-
CGQ03_RS13455	2553510..2553872	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
CGQ03_RS13460	2554127..2555377	+	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
CGQ03_RS13465	2555474..2556205	-	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
CGQ03_RS13470	2556202..2556921	-	720	WP_000479576.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T80069 WP_000113265.1 NZ_CP022905:c2552368-2552102 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T80069 NZ_CP022905:c2552368-2552102 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACAATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT80069 WP_000584499.1 NZ_CP022905:c2552619-2552368 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT80069 NZ_CP022905:c2552619-2552368 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60