Detailed information of TA system TA079793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 85051..85703
• Replicon: plasmid unnamed
• Accession: NZ_CP022795
• Organism: Ralstonia solanacearum strain SL2330

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A454TTN3
• Locus tag: CJO96_RS17410
• Protein ID: WP_038962847.1
• Coordinates: 85051..85395 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CJO96_RS17415
• Protein ID: WP_020425351.1
• Coordinates: 85392..85703 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJO96_RS17395	80746..81909	-	1164	WP_118872529.1	2-methylcitrate synthase	-
CJO96_RS17400	81961..82857	-	897	WP_038962849.1	methylisocitrate lyase	-
CJO96_RS17405	83051..85000	+	1950	WP_118872530.1	propionate catabolism operon regulatory protein PrpR	-
CJO96_RS17410	85051..85395	+	345	WP_038962847.1	hypothetical protein	Toxin
CJO96_RS17415	85392..85703	+	312	WP_020425351.1	transcriptional regulator	Antitoxin
CJO96_RS17420	85843..86652	-	810	WP_043885575.1	DUF2242 domain-containing protein	-
CJO96_RS17430	87905..88870	-	966	WP_016723619.1	IS5-like element IS1405 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cyaB / flgC / flgG / fliM / fliG / fliI / acrB / htpB / adeG / cheY / cheB / cheW / motA	1..2001855	2001855
	-	flank	IS/Tn	-	-	87905..88870	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13227.94 Da
• Isoelectric Point: 8.4489

>T79793 WP_038962847.1 NZ_CP022795:85051-85395 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIDYHWDGGGQFWM
FVVYDKGEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T79793 NZ_CP022795:85051-85395 [Ralstonia solanacearum]
ATGGATGCTACCTTTATCGAGTTGCCGCCTTTTCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACCGAGCGCT
CCAGCAGGAACTGCTCGCCCGGCCGGACGCCGGCGACGTCATCAAGGGGACAGGTGGTCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTGATTGACTACCACTGGGATGGAGGCGGCCAATTCTGGATG
TTTGTCGTCTACGACAAGGGCGAGGCGGACGACCTTTCCAGCGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT79793 WP_020425351.1 NZ_CP022795:85392-85703 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT79793 NZ_CP022795:85392-85703 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAGTTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGCGAAGGAAAGATGACCCTTCG
CAAGGTGAAGGTCCGCGTGCTCCAGCCGCTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCTT
CGCAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCTGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: A0A454TTN3