Detailed information of TA system TA079757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 127294..127946
• Replicon: plasmid unnamed
• Accession: NZ_CP022777
• Organism: Ralstonia solanacearum strain T11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G3AAL2
• Locus tag: CJO86_RS17300
• Protein ID: WP_075465943.1
• Coordinates: 127294..127638 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CJO86_RS17305
• Protein ID: WP_075465945.1
• Coordinates: 127635..127946 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJO86_RS17285	123017..124180	-	1164	WP_075465937.1	2-methylcitrate synthase	-
CJO86_RS17290	124215..125111	-	897	WP_082317449.1	methylisocitrate lyase	-
CJO86_RS17295	125306..127243	+	1938	WP_075465941.1	propionate catabolism operon regulatory protein PrpR	-
CJO86_RS17300	127294..127638	+	345	WP_075465943.1	toxin	Toxin
CJO86_RS17305	127635..127946	+	312	WP_075465945.1	transcriptional regulator	Antitoxin
CJO86_RS17310	128086..128913	-	828	WP_075468526.1	DUF2242 domain-containing protein	-
CJO86_RS17315	129237..129602	+	366	WP_075468528.1	hypothetical protein	-
CJO86_RS17320	129622..130221	+	600	WP_157771967.1	hypothetical protein	-
CJO86_RS17325	130243..130674	-	432	WP_075465948.1	hypothetical protein	-
CJO86_RS17330	130704..131036	-	333	WP_075465950.1	DUF5076 domain-containing protein	-
CJO86_RS17335	131691..132053	-	363	WP_075465952.1	hypothetical protein	-
CJO86_RS17340	132408..132890	-	483	WP_157771968.1	YcxB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / flgC / flgG / fliM / fliG / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1883805	1883805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13434.09 Da
• Isoelectric Point: 5.9519

>T79757 WP_075465943.1 NZ_CP022777:127294-127638 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLAQPDAGDVIRGTSGLRKLRFSDKRRGKGKRGGLRVIYYYWDEGGQFWM
FAVYDKDEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T79757 NZ_CP022777:127294-127638 [Ralstonia solanacearum]
ATGGATGCTACCTTTATTGAGTTGCCGCCTTTCCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACAGAGCACT
TCAACAGGAACTGCTTGCCCAGCCGGACGCCGGCGACGTCATCAGGGGGACAAGTGGTCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTCATTTACTACTACTGGGATGAAGGCGGCCAGTTCTGGATG
TTTGCCGTCTATGACAAGGACGAGGCGGACGACCTCTCCAGCGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11575.53 Da
• Isoelectric Point: 10.7132

>AT79757 WP_075465945.1 NZ_CP022777:127635-127946 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKTTLRKVKVRVLQPVDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT79757 NZ_CP022777:127635-127946 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAATTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGTGAAGGCAAGACGACCCTTCG
CAAGGTCAAGGTCCGCGTGCTCCAGCCGGTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCCT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGTACCTACCAGAATTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCAGTGTTGATCAAGCTCGTGGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: G3AAL2