Detailed information of TA system TA079746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 127275..127927
• Replicon: plasmid unnamed
• Accession: NZ_CP022771
• Organism: Ralstonia solanacearum strain T51

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G3AAL2
• Locus tag: CJO82_RS17065
• Protein ID: WP_075465943.1
• Coordinates: 127275..127619 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CJO82_RS17070
• Protein ID: WP_075465945.1
• Coordinates: 127616..127927 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJO82_RS17050	122998..124161	-	1164	WP_075465937.1	2-methylcitrate synthase	-
CJO82_RS17055	124196..125092	-	897	WP_082317449.1	methylisocitrate lyase	-
CJO82_RS17060	125287..127224	+	1938	WP_075465941.1	propionate catabolism operon regulatory protein PrpR	-
CJO82_RS17065	127275..127619	+	345	WP_075465943.1	toxin	Toxin
CJO82_RS17070	127616..127927	+	312	WP_075465945.1	transcriptional regulator	Antitoxin
CJO82_RS17075	128067..128894	-	828	WP_075468526.1	DUF2242 domain-containing protein	-
CJO82_RS17080	129218..129583	+	366	WP_075468528.1	hypothetical protein	-
CJO82_RS17085	129603..130202	+	600	WP_157771967.1	hypothetical protein	-
CJO82_RS17090	130224..130655	-	432	WP_075465948.1	hypothetical protein	-
CJO82_RS17095	130685..131017	-	333	WP_075465950.1	DUF5076 domain-containing protein	-
CJO82_RS17100	131673..132035	-	363	WP_075465952.1	hypothetical protein	-
CJO82_RS17105	132390..132872	-	483	WP_157771968.1	YcxB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / flgC / flgG / fliM / fliG / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1883756	1883756

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13434.09 Da
• Isoelectric Point: 5.9519

>T79746 WP_075465943.1 NZ_CP022771:127275-127619 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLAQPDAGDVIRGTSGLRKLRFSDKRRGKGKRGGLRVIYYYWDEGGQFWM
FAVYDKDEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T79746 NZ_CP022771:127275-127619 [Ralstonia solanacearum]
ATGGATGCTACCTTTATTGAGTTGCCGCCTTTCCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACAGAGCACT
TCAACAGGAACTGCTTGCCCAGCCGGACGCCGGCGACGTCATCAGGGGGACAAGTGGTCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTCATTTACTACTACTGGGATGAAGGCGGCCAGTTCTGGATG
TTTGCCGTCTATGACAAGGACGAGGCGGACGACCTCTCCAGCGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11575.53 Da
• Isoelectric Point: 10.7132

>AT79746 WP_075465945.1 NZ_CP022771:127616-127927 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKTTLRKVKVRVLQPVDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT79746 NZ_CP022771:127616-127927 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAATTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGTGAAGGCAAGACGACCCTTCG
CAAGGTCAAGGTCCGCGTGCTCCAGCCGGTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCCT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGTACCTACCAGAATTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCAGTGTTGATCAAGCTCGTGGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: G3AAL2