Detailed information of TA system TA079733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 99526..100178
• Replicon: plasmid unnamed
• Accession: NZ_CP022764
• Organism: Ralstonia solanacearum strain T82

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G3AAL2
• Locus tag: CJO79_RS16855
• Protein ID: WP_075465943.1
• Coordinates: 99526..99870 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CJO79_RS16860
• Protein ID: WP_075465945.1
• Coordinates: 99867..100178 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJO79_RS16840	95249..96412	-	1164	WP_118884172.1	2-methylcitrate synthase	-
CJO79_RS16845	96447..97343	-	897	WP_118884974.1	methylisocitrate lyase	-
CJO79_RS16850	97538..99475	+	1938	WP_118884173.1	propionate catabolism operon regulatory protein PrpR	-
CJO79_RS16855	99526..99870	+	345	WP_075465943.1	toxin	Toxin
CJO79_RS16860	99867..100178	+	312	WP_075465945.1	transcriptional regulator	Antitoxin
CJO79_RS16865	100318..101145	-	828	WP_075468526.1	DUF2242 domain-containing protein	-
CJO79_RS24710	101450..101599	+	150	WP_162899627.1	hypothetical protein	-
CJO79_RS16870	101589..101996	+	408	WP_162899629.1	hypothetical protein	-
CJO79_RS16875	102237..102977	-	741	WP_118884175.1	metallophosphatase family protein	-
CJO79_RS16880	103206..105044	+	1839	WP_118884176.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / flgC / flgG / fliM / fliG / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1988569	1988569

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13434.09 Da
• Isoelectric Point: 5.9519

>T79733 WP_075465943.1 NZ_CP022764:99526-99870 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLAQPDAGDVIRGTSGLRKLRFSDKRRGKGKRGGLRVIYYYWDEGGQFWM
FAVYDKDEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T79733 NZ_CP022764:99526-99870 [Ralstonia solanacearum]
ATGGATGCTACCTTTATTGAGTTGCCGCCTTTCCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACAGAGCACT
TCAACAGGAACTGCTTGCCCAGCCGGACGCCGGCGACGTCATCAGGGGGACAAGTGGTCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTCATTTACTACTACTGGGATGAAGGCGGCCAGTTCTGGATG
TTTGCCGTCTACGACAAGGACGAGGCGGACGATCTCTCCAGCGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11575.53 Da
• Isoelectric Point: 10.7132

>AT79733 WP_075465945.1 NZ_CP022764:99867-100178 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKTTLRKVKVRVLQPVDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT79733 NZ_CP022764:99867-100178 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAATTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGTGAAGGCAAGACGACCCTTCG
CAAGGTCAAGGTCCGCGTGCTCCAGCCGGTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCCT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGTACCTACCAGAATTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCAGTGTTGATCAAGCTCGTGGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: G3AAL2