Detailed information of TA system TA079722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 99523..100175
• Replicon: plasmid unnamed
• Accession: NZ_CP022758
• Organism: Ralstonia solanacearum strain T101

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G3AAL2
• Locus tag: CJO76_RS16865
• Protein ID: WP_075465943.1
• Coordinates: 99523..99867 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CJO76_RS16870
• Protein ID: WP_075465945.1
• Coordinates: 99864..100175 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CJO76_RS16850	95246..96409	-	1164	WP_118884172.1	2-methylcitrate synthase	-
CJO76_RS16855	96444..97340	-	897	WP_118884974.1	methylisocitrate lyase	-
CJO76_RS16860	97535..99472	+	1938	WP_118884173.1	propionate catabolism operon regulatory protein PrpR	-
CJO76_RS16865	99523..99867	+	345	WP_075465943.1	toxin	Toxin
CJO76_RS16870	99864..100175	+	312	WP_075465945.1	transcriptional regulator	Antitoxin
CJO76_RS16875	100315..101142	-	828	WP_075468526.1	DUF2242 domain-containing protein	-
CJO76_RS24715	101447..101596	+	150	WP_162899627.1	hypothetical protein	-
CJO76_RS16880	101628..101993	+	366	WP_134940909.1	hypothetical protein	-
CJO76_RS16885	102234..102974	-	741	WP_118884175.1	metallophosphatase family protein	-
CJO76_RS16890	103203..105041	+	1839	WP_118884176.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / flgC / flgG / fliM / fliG / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..1988531	1988531

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13434.09 Da
• Isoelectric Point: 5.9519

>T79722 WP_075465943.1 NZ_CP022758:99523-99867 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLAQPDAGDVIRGTSGLRKLRFSDKRRGKGKRGGLRVIYYYWDEGGQFWM
FAVYDKDEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T79722 NZ_CP022758:99523-99867 [Ralstonia solanacearum]
ATGGATGCTACCTTTATTGAGTTGCCGCCTTTCCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACAGAGCACT
TCAACAGGAACTGCTTGCCCAGCCGGACGCCGGCGACGTCATCAGGGGGACAAGTGGTCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTCATTTACTACTACTGGGATGAAGGCGGCCAGTTCTGGATG
TTTGCCGTCTACGACAAGGACGAGGCGGACGATCTCTCCAGCGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11575.53 Da
• Isoelectric Point: 10.7132

>AT79722 WP_075465945.1 NZ_CP022758:99864-100175 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKTTLRKVKVRVLQPVDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT79722 NZ_CP022758:99864-100175 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAATTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGTGAAGGCAAGACGACCCTTCG
CAAGGTCAAGGTCCGCGTGCTCCAGCCGGTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCCT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGTACCTACCAGAATTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCAGTGTTGATCAAGCTCGTGGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: G3AAL2