Detailed information of TA system TA079701

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 569197..569812
• Replicon: chromosome
• Accession: NZ_CP022742
• Organism: Vibrio qinghaiensis strain Q67

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q9KMG5
• Locus tag: CCZ37_RS15875
• Protein ID: WP_001162670.1
• Coordinates: 569525..569812 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CCZ37_RS15870
• Protein ID: WP_026027241.1
• Coordinates: 569197..569514 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CCZ37_RS15840	565274..566140	+	867	WP_088728983.1	hypothetical protein	-
CCZ37_RS15845	566581..566901	+	321	WP_094501461.1	IS66 family insertion sequence hypothetical protein	-
CCZ37_RS15850	566898..567251	+	354	WP_094499614.1	IS66 family insertion sequence element accessory protein TnpB	-
CCZ37_RS15855	567311..568843	+	1533	WP_094499631.1	IS66 family transposase	-
CCZ37_RS19225	569090..569200	-	111	WP_082798255.1	DUF3265 domain-containing protein	-
CCZ37_RS15870	569197..569514	-	318	WP_026027241.1	HigA family addiction module antidote protein	Antitoxin
CCZ37_RS15875	569525..569812	-	288	WP_001162670.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CCZ37_RS15890	570130..571098	+	969	WP_094501471.1	IS30 family transposase	-
CCZ37_RS15895	571124..572107	-	984	WP_094501473.1	hypothetical protein	-
CCZ37_RS15900	572104..572643	-	540	WP_094501475.1	hypothetical protein	-
CCZ37_RS15910	573250..573360	-	111	WP_080115560.1	DUF3265 domain-containing protein	-
CCZ37_RS15915	573357..573680	-	324	WP_004397191.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	566581..571098	4517
	-	inside	Integron	-	-	569077..720478	151401

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11349.95 Da
• Isoelectric Point: 6.7187

>T79701 WP_001162670.1 NZ_CP022742:c569812-569525 [Vibrio qinghaiensis]
MALEFKDKWLEQFYEDDKRHRLIPSSIENALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHKY

Nucleotide

• Length: 288 bp

>T79701 NZ_CP022742:c569812-569525 [Vibrio qinghaiensis]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCTAGTAGCAT
AGAGAACGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCACCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAAGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11719.66 Da
• Isoelectric Point: 10.4711

>AT79701 WP_026027241.1 NZ_CP022742:c569514-569197 [Vibrio qinghaiensis]
MRKTKRRPVSVGEMLKIEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT79701 NZ_CP022742:c569514-569197 [Vibrio qinghaiensis]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAATTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCTATGGGCGTACACAGAAATACGGTGAGTAATTTGATTAATGGCGGAGTGTTAACAGCTCCGG
TAGCAATCAAGTTGGCCGCGGCATTAGGTAATACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA

Structures

Toxin

• AlphaFold DB: A0A0F2HI36