Detailed information of TA system TA079688

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 91031..91300
• Replicon: plasmid pSA186_3
• Accession: NZ_CP022732
• Organism: Escherichia coli strain SA186

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CHQ92_RS25155
• Protein ID: WP_001312861.1
• Coordinates: 91142..91300 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	91031..91096 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHQ92_RS25130	86840..87337	+	498	WP_089552049.1	single-stranded DNA-binding protein	-
CHQ92_RS25135	87400..87633	+	234	WP_000005990.1	DUF905 family protein	-
CHQ92_RS25140	87699..89657	+	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
CHQ92_RS25145	89712..90146	+	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
CHQ92_RS25150	90143..90862	+	720	WP_001276228.1	plasmid SOS inhibition protein A	-
CHQ92_RS28115	90874..91062	-	189	WP_001299721.1	hypothetical protein	-
-	90874..91098	+	225	NuclAT_0	-	-
-	90874..91098	+	225	NuclAT_0	-	-
-	90874..91098	+	225	NuclAT_0	-	-
-	90874..91098	+	225	NuclAT_0	-	-
-	91031..91096	+	66	-	-	Antitoxin
CHQ92_RS25155	91142..91300	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CHQ92_RS28260	91991..92197	+	207	WP_000547939.1	hypothetical protein	-
CHQ92_RS25175	92222..92509	+	288	WP_000107535.1	hypothetical protein	-
CHQ92_RS25180	92631..93452	+	822	WP_001234469.1	DUF945 domain-containing protein	-
CHQ92_RS25185	93749..94351	-	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
CHQ92_RS25190	94672..95055	+	384	WP_053320747.1	relaxosome protein TraM	-
CHQ92_RS25195	95242..95931	+	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / aac(3)-IId	iroN / iroE / iroD / iroC / iroB	1..113162	113162

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T79688 WP_001312861.1 NZ_CP022732:91142-91300 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T79688 NZ_CP022732:91142-91300 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT79688 NZ_CP022732:91031-91096 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin