Detailed information of TA system TA079683

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 168481..168906
• Replicon: plasmid pSA186_2
• Accession: NZ_CP022731
• Organism: Escherichia coli strain SA186

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: CHQ92_RS24460
• Protein ID: WP_001332444.1
• Coordinates: 168745..168906 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	168481..168692 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHQ92_RS24430	164455..164952	+	498	WP_050427786.1	single-stranded DNA-binding protein	-
CHQ92_RS24435	165015..165248	+	234	WP_001209608.1	DUF905 domain-containing protein	-
CHQ92_RS24440	165307..167265	+	1959	Protein_161	ParB/RepB/Spo0J family partition protein	-
CHQ92_RS24445	167320..167754	+	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
CHQ92_RS24450	167751..168470	+	720	WP_001276275.1	plasmid SOS inhibition protein A	-
-	168481..168692	+	212	NuclAT_0	-	Antitoxin
-	168481..168692	+	212	NuclAT_0	-	Antitoxin
-	168481..168692	+	212	NuclAT_0	-	Antitoxin
-	168481..168692	+	212	NuclAT_0	-	Antitoxin
CHQ92_RS24460	168745..168906	+	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
CHQ92_RS24465	169144..169756	-	613	Protein_165	hypothetical protein	-
CHQ92_RS24470	169935..170756	+	822	Protein_166	DUF932 domain-containing protein	-
CHQ92_RS24475	171052..171642	-	591	WP_000252676.1	transglycosylase SLT domain-containing protein	-
CHQ92_RS24485	171976..172359	+	384	WP_000124981.1	relaxosome protein TraM	-
CHQ92_RS24490	172549..173235	+	687	WP_000332490.1	PAS domain-containing protein	-
CHQ92_RS24495	173329..173556	+	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA	1..198748	198748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T79683 WP_001332444.1 NZ_CP022731:168745-168906 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T79683 NZ_CP022731:168745-168906 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 212 bp

>AT79683 NZ_CP022731:168481-168692 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin