Detailed information of TA system TA079670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2716383..2716985
• Replicon: chromosome
• Accession: NZ_CP022730
• Organism: Escherichia coli strain SA186

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: CHQ92_RS13400
• Protein ID: WP_000897302.1
• Coordinates: 2716383..2716694 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CHQ92_RS13405
• Protein ID: WP_000356397.1
• Coordinates: 2716695..2716985 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CHQ92_RS13375	2712297..2712896	+	600	WP_001296610.1	glucose-1-phosphatase	-
CHQ92_RS13380	2712890..2713762	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CHQ92_RS13385	2713759..2714196	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CHQ92_RS13390	2714241..2715182	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CHQ92_RS13395	2715246..2716154	-	909	WP_001385591.1	alpha/beta hydrolase	-
CHQ92_RS13400	2716383..2716694	+	312	WP_000897302.1	hypothetical protein	Toxin
CHQ92_RS13405	2716695..2716985	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CHQ92_RS13410	2717070..2717282	+	213	WP_000197774.1	hypothetical protein	-
CHQ92_RS13415	2717344..2717622	+	279	WP_001296612.1	hypothetical protein	-
CHQ92_RS13425	2718019..2718237	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
CHQ92_RS13430	2718422..2718871	-	450	WP_032140890.1	hypothetical protein	-
CHQ92_RS13435	2719187..2720035	-	849	WP_001038650.1	hypothetical protein	-
CHQ92_RS13440	2720325..2720567	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
CHQ92_RS13445	2720749..2721678	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T79670 WP_000897302.1 NZ_CP022730:2716383-2716694 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T79670 NZ_CP022730:2716383-2716694 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT79670 WP_000356397.1 NZ_CP022730:2716695-2716985 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT79670 NZ_CP022730:2716695-2716985 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416